Impact of Fusobacterium nucleatum in the gastrointestinal tract on natural killer cells

作     者：Yeon Ji Kim Bu Kyung Kim Seun Ja Park Jae Hyun Kim 

作者机构：Institute of GastroenterologyKosin University College of MedicineBusan 49267Spain Department of Internal MedicineKosin University College of MedicineBusan 49267South Korea 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2021年第27卷第29期

页      面：4879-4889页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：Supported by National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT) No.2017R1C1B5017576 

主　　题：Fusobacterium nucleatum Natural killer cells Colorectal cancer 

摘      要：BACKGROUND Gut microbial dysbiosis contributes to the development and progression of colorectal cancer(CRC).Natural killer(NK)cells are involved in early defense mechanisms to kill infective pathogens and tumor cells by releasing chemokines and *** better understand the relationship between the gut microbiome and CRC,it was hypothesized here that a high abundance of Fusobacterium nucleatum(***)in the gastrointestinal tract could cause reduced NK cell *** To identify associations between gastrointestinal tract *** levels and NK cell *** In vitro experiments were performed on NK cells treated with ***,Peptostreptococcus anaerobius,and Parvimonas micra to identify the effects of gut microbiome species on NK *** 24 and 48 h of treatment,NK cell counts were *** parallel studies,C57BL/6 mice were given broadspectrum antibiotics in their drinking water to reduce resident gut *** 3 wk,the mice received the various bacterial species or phosphate-buffered saline(PBS)via oral gavage every 2 d for 6 *** the study end,blood samples were acquired to perform NK cell activity assessment and cytokine *** tissues were collected and analyzed via immunohistochemistry(IHC).RESULTS The data show that after 3 wk of broad-spectrum antibiotic treatment,levels of total bacteria and *** were markedly decreased in *** of *** significantly decreased NK cell activity relative to the activities of cells from mice treated with antibiotics only and *** administration of *** decreased the proportion of NK46+cells based on IHC staining and increased the production of interleukin-1βand tumor necrosis factor-α.CONCLUSION High levels of *** in the gastrointestinal tract reduced NK cell activity in mice,and the decrease in NK cell activity might be affected by increased proinflammatory cytokines after *** treatment.