The microbiota is dispensable for the early stages of peripheral regulatory T cell induction within mesenteric lymph nodes

作     者：Carolin Wiechers Mangge Zou Eric Galvez Michael Beckstette Maria Ebel Till Strowig Jochen Huehn Joern Pezoldt 

作者机构：Department Experimental ImmunologyHelmholtz Centre for Infection ResearchBraunschweigGermany Department Microbial Immune RegulationHelmholtz Centre for Infection ResearchBraunschweigGermany Department of Computational Biology for Individualised MedicineCentre for Individualised Infection MedicineHelmholtz Centre for Infection Research and Hannover Medical SchoolHannoverGermany Cluster of Excellence RESIST(EXC 2155)Hannover Medical SchoolHannoverGermany Laboratory of Systems Biology and GeneticsÉcole Polytechnique Fédérale de LausanneLausanneSwitzerland 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2021年第18卷第5期

页      面：1211-1221页

核心收录：

学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the Helmholtz-Gemeinschaft(Zukunftsthema“Aging and Metabolic Reprogramming”,ZT-0026) the Ministry for Science and Culture of Lower Saxony(research consortium COALITION) the Deutsche Forschungsgemeinschaft(SPP1656,PE 2840/1-1,and Germany’s Excellence Strategy-EXC 2155“RESIST”-Project ID 39087428). 

主　　题：Peripheral regulatory T cells Microbiota Tolerance 

摘      要：Intestinal Foxp3+regulatory T cell(Treg)subsets are crucial players in tolerance to microbiota-derived and food-borne antigens,and compelling evidence suggests that the intestinal microbiota modulates their generation,functional specialization,and maintenance.Selected bacterial species and microbiota-derived metabolites,such as short-chain fatty acids(SCFAs),have been reported to promote Treg homeostasis in the intestinal lamina propria.Furthermore,gut-draining mesenteric lymph nodes(mLNs)are particularly efficient sites for the generation of peripherally induced Tregs(pTregs).Despite this knowledge,the direct role of the microbiota and their metabolites in the early stages of pTreg induction within mLNs is not fully elucidated.Here,using an adoptive transfer-based pTreg induction system,we demonstrate that neither transfer of a dysbiotic microbiota nor dietary SCFA supplementation modulated the pTreg induction capacity of mLNs.Even mice housed under germ-free(GF)conditions displayed equivalent pTreg induction within mLNs.Further molecular characterization of these de novo induced pTregs from mLNs by dissection of their transcriptomes and accessible chromatin regions revealed that the microbiota indeed has a limited impact and does not contribute to the initialization of the Treg-specific epigenetic landscape.Overall,our data suggest that the microbiota is dispensable for the early stages of pTreg induction within mLNs.