Mutations associated with egg adaptation of influenza A(H1N1)pdm09 virus in laboratory based surveillance in China, 2009–2016

作     者：Lei Yang Yanhui Cheng Xiang Zhao Hejiang Wei Minju Tan Xiyan Li Wenfei Zhu Weijuan Huang Wenbing Chen Jia Liu Zi Li Yuelong Shu Dayan Wang Yang Lei;Cheng Yanhui;Zhao Xiang;Wei Hejiang;Tan Minju;Li Xiyan;Zhu Wenfei;Huang Weijuan;Chen Wenbing;Liu Jia;Li Zi;Shu Yuelong;Wang Dayan

作者机构：National Institute for Viral Disease Control and PreventionChina CDCKey Laboratory for Medical VirologyNational Health CommissionBeijingPeople's Republic of China School of Public Health(Shenzhen)Sun Yat-sen UniversityGuangdongPeople's Republic of China 

出 版 物：《Biosafety and Health》 (生物安全与健康（英文）)

年 卷 期：2019年第1卷第1期

页      面：41-45页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 02[经济学] 0202[经济学-应用经济学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0836[工学-生物工程] 

基　　金：supported by the National Key Research and Development Program of China(2016YFD0500208 to Dayan Wang and 2016YFC1200200 to Yuelong Shu) the National Mega-projects for Infectious Diseases(2017ZX10303401-004). 

主　　题：Influenza A(H1N1)pdm09 virus Surveillance Egg-adapted mutation 

摘      要：Mutations of influenza virus associated with adaptation occurring during passage in embryonated chicken eggs could result in antigenic change or reduced vaccine effectiveness.In this study,we investigated the mutations of influenza A(H1N1)pdm09 egg isolates from the Chinese National Influenza Surveillance Network between 2009 and 2016.Thirteen mutations were identified in the hemagglutinin(HA)protein from viruses passaged in eggs,in comparing to those in cells.After scanning public database,four mutations,D127E,L191I,D222G/N and Q223R in HA1,which may alter the receptor-binding specificity,were observed frequently.Although the A(H1N1)pdm09 virus has evolved in human for nearly ten years,most egg-cultured viruses acquired one or more further mutations.Using the egg isolates for influenza surveillance requires extra caution because of these selected mutations,and their impacts on antigenicity and receptor-binding property need further evaluation.Currently,most of the influenza vaccines are produced using egg isolates,particularly in China.Thus,there is an urge to promote the establishment of an alternative influenza vaccine production platform.