Use of DNA metabarcoding of bird pellets in understanding raptor diet on the Qinghai-Tibetan Plateau of China

作     者：Charlotte E.Hacker Brandon D.Hoenig Liji Wu Wei Cong Jingjing Yu Yunchuan Dai Ye Li Jia Li Yadong Xue Yu Zhang Yunrui Ji Hanning Cao Diqiang Li Yuguang Zhang Jan E.Janecka Charlotte E.Hacker;Brandon D.Hoenig;Liji Wu;Wei Cong;Jingjing Yu;Yunchuan Dai;Ye Li;Jia Li;Yadong Xue;Yu Zhang;Yunrui Ji;Hanning Cao;Diqiang Li;Yuguang Zhang;Jan E.Janecka

作者机构：Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of Forestryand Key Laboratory of Biodiversity Protection of National Forestry and Grassland AdministrationBeijing 100091China Department of Biological SciencesDuquesne UniversityPittsburghPA 15282USA Yanchiwan National Nature Reserve of Gansu ProvinceSubei 736300China Institute for Ecology and Environmental ResourcesChongqing Academy of Social SciencesChongqing 400020China The High School Affiliated to RenminUniversity of ChinaBeijing 100872China 

出 版 物：《Avian Research》 (鸟类学研究（英文版）)

年 卷 期：2021年第12卷第3期

页      面：296-306页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：funded by the National Key Technology R&D Program of China(2019YFA0607103) Snow Leopard Conservancy(G1900014,G2000019) Cleveland Metroparks Zoo(G1800082) Panthera Corporation and The Andrew Sabin Family Foundation(G1900011,G2000017) Welfare Project of the National Scientific Research Institution(CAFYBB2019ZE003) 

主　　题：Avian Eurasian Eagle Owl Molecular diet analysis Next-generation sequencing Raptor Saker Falcon Steppe Eagle Upland Buzzard 

摘      要：Background:Diet analysis is essential to understanding the functional role of large bird species in food ***-phological analysis of regurgitated bird pellet contents is time intensive and may underestimate *** metabarcoding has the ability to circumvent these issues,but has yet to be ***:We present a pilot study using DNA metabarcoding of MT-RNR1 and MT-CO1 markers to determine the species of origin and prey of 45 pellets collected in Qinghai and Gansu Provinces,***:We detected four raptor species[Eurasian Eagle Owl(Bubo bubo),Saker Falcon(Falco cherrug),Steppe Eagle(Aquila nipalensis),and Upland Buzzard(Buteo hemilasius)]and 11 unique prey species across 10 families and 4 *** were the greatest detected prey class with Plateau Pika(Ochotona curzoniae)being the most *** Shannon’s and Simpson’s diversity for Upland Buzzard were 1.089 and 0.479,respectively,while expected values were 1.312±0.266 and 0.485±*** Eurasian Eagle Owl,observed values were 1.202 and 0.565,while expected values were 1.502±0.340 and 0.580±*** dietary niche partitioning between the two spe-cies was not ***:Our results demonstrate successful use of DNA metabarcoding for understanding diet via a novel noninvasive sample type to identify common and uncommon *** work is needed to understand how raptor diets vary locally,and the mechanisms that enable exploitation of similar dietary *** approach has wide ranging applicability to other birds of prey,and demonstrates the power of using DNA metabarcoding to study species noninvasively.