Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance

作     者：Yang Yong Lin Qiujun Chen Xinyu Liang Weifang Fu Yuwen Xu Zhengjin Wu Yuanhua Wang Xuming Zhou Jie Yu Chulang Yan Chengqi Mei Qiong Chen Jianping YANG Yong;LIN Qiujun;CHEN Xinyu;LIANG Weifang;FU Yuwen;XU Zhengjin;WU Yuanhua;WANG Xuming;ZHOU Jie;YU Chulang;YAN Chengqi;MEI Qiong;CHEN Jianping

作者机构：State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Key Laboratory of Biotechnology far Plant ProtectionMinistry of Agriculture and Rural Affairs of China/Zhejiang Provincial Key Laboratory of Biotechnology far Plant ProtectionInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural ScienceHangzhou 310021China Plant Pathogen LaboratoryCollege of Plant ProtectionShenyang Agricultural UniversityShenyang 110866China State Key Laboratory of Subtropical SilvicultureZhejiang Agriculture and Forest UniversityHangzhou 311300China College of Plant ProtectionYunnan Agricultural UniversityKunming 650201China State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Key Laboratory of Biotechnology for Plant ProtectionMinistry of Agriculture and Rural Affairs of China/Zhejiang Provincial Key Laboratory of Biotechnology for Plant ProtectionInstitute of Plant VirologyNingbo UniversityNingbo 315211China institute of BiotechnologyNingbo Academy of Agricultural ScienceNingbo 315040China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2021年第28卷第5期

页      面：466-478,I0020-I0028页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the Zhejiang Provincial Key Research and Development Plan(Grant No.2019C02006) the National Key Research and Development Program of China(Grant Nos.2016YFD0200804 and 2016YFD0100601-15) the Key Program of Zhejiang Provincial Foundation for Natural Science(Grant No.LZ16C130002) the Zhejiang Fundamental Public Welfare Research Program(Grant No.LGN19C140008) the State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products(Grant No.2010DS700124-ZZ1907) 

主　　题：lesion mimic muta nt H_(2)O_(2) disease resista nee pathoge nesis・related protein resista nce・related pathway 

摘      要：Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to ***,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice *** phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome *** data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were *** proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type *** expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/*** proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil *** of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates *** resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with *** results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.