Investigation of the Antimicrobial Activity and <i>in Vivo </i>Cytotoxicity of <i>Diospyros malabarica </i>(Desr.) Kostel. Fruit Extracts

作     者：Razib Datta Shubhra Shakil Ahmed Polash Md. Monir Hossain Amir Hamza Md. Mehedi Hasan Tushar Tanushree Saha Md. Ashraful Hasan Md. Maniruzzaman Sikder Nuhu Alam Zinia Islam Md. Sharif Hossain Satya Ranjan Sarker Razib Datta Shubhra;Shakil Ahmed Polash;Md. Monir Hossain;Amir Hamza;Md. Mehedi Hasan Tushar;Tanushree Saha;Md. Ashraful Hasan;Md. Maniruzzaman Sikder;Nuhu Alam;Zinia Islam;Md. Sharif Hossain;Satya Ranjan Sarker

作者机构：Department of Biotechnology and Genetic Engineering Jahangirnagar University Savar Bangladesh Department of Textile Engineering Dhaka University of Engineering and Technology Gazipur Bangladesh Department of Biochemistry and Molecular Biology Jahangirnagar University Savar Bangladesh Department of Botany Jahangirnagar University Savar Bangladesh 

出 版 物：《Natural Science》 (自然科学期刊（英文）)

年 卷 期：2021年第13卷第8期

页      面：331-351页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Diospyros malabarica (Desr.) Kostel. Antibacterial Activity Trypan Blue Assay Cell ToxTM Green Assay In Vivo Cytotoxicity 

摘      要：Mankind is facing an unprecedented threat of existence due to the antibiotic resistance developed by bacteria. The unripe fruits of Diospyros malabarica (Desr.) Kostel. (family: Ebenaceae) can be considered as one of the natural sources to tackle this issue. The present study is designed to assess the antimicrobial activity of D. malabarica seed and flesh ex-tracts. Herein, D. malabarica extracts were prepared using polar solvents (i.e., water and 70% ethanol) and their antimicrobial activity as well as in vivo toxicity was investigated. Their antibacterial activity was investigated against gram positive (Bacillus subtilis) and gram negative (Escherichia coli DH5α, and Salmonella typhi) bacteria at different time points. All the extracts showed the highest antibacterial activity after 2 hours of incubation. The aqueous seed extract showed the maximum zone of inhibition (i.e., ~13 mm) against Bacillus subtilis with a minimum inhibitory concentration (MIC) value of 2 μg/μl. The an-tibacterial propensity was also confirmed through trypan blue dye exclusion assay, CellToxTM Green assay, and lipid peroxidation (LPO) assay. On the other hand, the etha-nolic seed extract demonstrated higher antifungal activity through inhibition of mycelial growth. All the extracts showed excellent hemocompatibility against both human and rat red blood cells (RBCs). They also did not show any toxicity to rat liver and kidneys. Taken together, this study demonstrates that the aqueous and ethanolic extracts of D. malabarica seed and flesh could be an effective source of natural antimicrobial agents with no cytotox-ic activity.