Alternatively spliced BobCAL transcripts alter curd morphotypes in a collection of Chinese cauliflower accessions
作者机构：National Laboratory of Plant Molecular GeneticsCenter for Excellence in Molecular Plant SciencesShanghai Institutes of Plant Physiology&EcologyChinese Academy of Science300 Fenglin RoadShanghai 200032China State Key Laboratory of Crop Stress Biology in Arid Areas and College of AgronomyNorthwest A&F UniversityYanglingShaanxiChina State Key Laboratory of North China Crop Improvement and RegulationDepartment of HorticultureHebei Agricultural UniversityBaoding 071001China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2020年第7卷第1期
页 面：649-661页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0836[工学-生物工程] 090202[农学-蔬菜学] 0834[工学-风景园林学（可授工学、农学学位）]
基 金：supported by the National Programs for Science and Technology Development of China(Grant Nos.2016YFD0101900 and 2016YFD100500) the Natural Science Foundation of China(Grant No.31571261) the Shanghai Agriculture Applied Technology Development Program,China(Grant Nos.G2015060107 and Z20160109).
主 题：collection retention floral
摘 要：The curd of cauliflower(Brassica oleracea L.var.botrytis)is a modified inflorescence that is consumed as a vegetable.Curd formation is proposed to be due to a mutation in the BobCAULIFLOWER(BobCAL)gene,but the genetic relationship between BobCAL variation and curd morphotypes remains obscure.To address this question,we collected and classified a collection of 78 cauliflower accessions into four subpopulations according to curd surface features:smooth,coarse,granular,and hairy curd morphotypes.Through the cDNA sequencing of BobCAL alleles,we showed that smooth and coarse accessions characterized by inflorescence meristem arrest presented a strong association with the 451T SNP(BobCAL_T),whereas granular and hairy accessions marked with floral organ arrest presented an association with 451G(BobCAL_G).Interestingly,all BobCAL alleles were alternatively spliced,resulting in a total of four alternative splice(AS)variants due to the retention of the fourth and/or seventh introns.Among accessions with BobCAL_G alleles,the total expression of all these AS variants in granular plants was almost equal to that in hairy plants;however,the expression of the individual AS variants encoding intact proteins relative to those encoding truncated proteins differed.Hairy accessions showed relatively high expression of the individual variants encoding intact proteins,whereas granular accessions displayed relatively low expression.In smooth cauliflower,the overexpression of the BobCAL_Ga variant caused an alteration in the curd morphotype from smooth to hairy,concurrent with an increase in the expression levels of downstream floral identity genes.These results reveal that alternative splicing of BobCAL transcripts is involved in the determination of cauliflower curd morphotypes.
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