Engineering microscale systems for fully autonomous intracellular neural interfaces
作者机构：Biomedical EngineeringSchool of Biological and Health Systems EngineeringArizona State UniversityTempeAZ 85287USA Mechanical EngineeringSandia National laboratoriesAlbuquerqueNMUSA mPower TechnologyInc.AlbuquerqueNM 87102USA
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2020年第6卷第1期
页 面：1276-1295页
核心收录：
学科分类：0711[理学-系统科学] 0808[工学-电气工程] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0811[工学-控制科学与工程] 0702[理学-物理学]
基 金：This research was supported by the NIH R21NS084492-01 grant。
主 题：recording electrode system
摘 要：Conventional electrodes and associated positioning systems for intracellular recording from single neurons in vitro and in vivo are large and bulky,which has largely limited their scalability.Further,acquiring successful intracellular recordings is very tedious,requiring a high degree of skill not readily achieved in a typical laboratory.We report here a robotic,MEMS-based intracellular recording system to overcome the above limitations associated with form factor,scalability,and highly skilled and tedious manual operations required for intracellular recordings.This system combines three distinct technologies:(1)novel microscale,glass–polysilicon penetrating electrode for intracellular recording;(2)electrothermal microactuators for precise microscale movement of each electrode;and(3)closed-loop control algorithm for autonomous positioning of electrode inside single neurons.Here we demonstrate the novel,fully integrated system of glass–polysilicon microelectrode,microscale actuators,and controller for autonomous intracellular recordings from single neurons in the abdominal ganglion of Aplysia californica(n=5 cells).Consistent resting potentials(60 mV)were recorded after each successful penetration attempt with the controller and microactuated glass–polysilicon microelectrodes.The success rate of penetration and quality of intracellular recordings achieved using electrothermal microactuators were comparable to that of conventional positioning systems.Preliminary data from in vivo experiments in anesthetized rats show successful intracellular recordings.The MEMS-based system offers significant advantages:(1)reduction in overall size for potential use in behaving animals,(2)scalable approach to potentially realize multi-channel recordings,and(3)a viable method to fully automate measurement of intracellular recordings.This system will be evaluated in vivo in future rodent studies.
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