Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

作     者：MO Ji-hao XIE Han-kun ZHOU Ye-mian Ng Sihan-benjamin LI Shao-xia WANG Lei MO Ji-hao;XlE Han-kun;ZHOU Ye-mian;Ng Sihan-benjamin;LI Shao-xia;WANG Lei

作者机构：Department of Medical LaboratoryLuoyang Orthopedic Hospital of Henan ProvinceOrthopedic Institute of Henan ProvinceLuoyangHenan Province(471002)China Miller School of MedicineUniversity of Miami(33136)MiamiFLUSA Institute of Technical Biology&Agriculture EngineeringHefei Institutes of Physical ScienceChinese Academy of SciencesHefei(230031)China Eatobe Pte.Limited(573969)Singapore 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2021年第27卷第10期

页      面：767-773页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：rheumatoid arthritis estrogen receptor 1 inokosterone Cyathula Officinalis Chinese medicine 

摘      要：Objective:To elucidate the active compounds and the molecular mechanism of Cyathula Off/c/na//s as a drug treatment for rheumatoid arthritis(RA).Methods:The target genes of active ingredients from Cyathula Officinalis were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese *** protein-protein interaction between the target genes were analyzed using STRING and *** transcriptome of RA patients compared to healthy people(GSE121894)were analyzed using R program package *** relative expression of the target genes was obtained from the RNA-seq *** molecular docking analyses were processed based on the molecular model of estrogen receptor 1(ESR1)binding with estradiol(PDB ID:1A52).The binding details were analyzed by ***:Inokosterone,ecdysterone,and cyaterone were the 3 active ingredients from Cyathula Officinalis that bind to target *** all the significantly changed genes from RA patients,ESR1,ADORA1,and ANXA1 were significantly increased in mRNA samples of RA ***:ESR1,the transcription factor that binds inokosterone in the molecular binding analysis,is the target protein of Cyathula Officinalis.