Regulations of m^(6)A methylation on tomato fruit chilling injury

作     者：Chunmei Bai Minghuan Fang Baiqiang Zhai Lili Ma Anzhen Fu Lipu Gao Xiaohong Kou Demei Meng Qing Wang Shufang Zheng Jinhua Zuo Chunmei Bai;Minghuan Fang;Baiqiang Zhai;Lili Ma;Anzhen Fu;Lipu Gao;Xiaohong Kou;Demei Meng;Qing Wang;Shufang Zheng;Jinhua Zuo

作者机构：State Key Laboratory of Food Nutrition and SafetyCollege of Food Science and EngineeringTianjin University of Science&TechnologyTianjin 300457China Key Laboratory of Vegetable Postharvest ProcessingMinistry of AgricultureBeijing Key Laboratory of Fruit and Vegetable Storage and ProcessingKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)of Ministry of AgricultureKey Laboratory of Urban Agriculture(North)of Ministry of AgricultureBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Key Laboratory of Marine Chemistry Theory and TechnologyMinistry of EducationOcean University of ChinaQingdao 266100China Henan Railway Food Safety Management Engineering Technology Research CenterZhengzhou 451460China College of Food Science and TechnologyHebei Agricultural UniversityBaoding 071001China School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2021年第7卷第5期

页      面：434-442页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant Nos.31772022,32072284 and 31501544) the Special Innovation Ability Construction Fund of Beijing Academy of Agricultural and Forestry Sciences(Grant Nos.20200427 and 20210437) Science and Technology Planning Project of Tianjin City(Grant No.19YFSLQY00100) the Beijing Municipal Science and Technology Commission(Grant Nos.Z191100008619004 and Z191100004019010) Supported by China Agriculture Research System of MOF and MARA,collaborative innovation center of Beijing Academy of Agricultural and Forestry Sciences(Grant No.201915) the Young Investigator Fund of Beijing Academy of Agricultural and Forestry Sciences(Grant No.202016) the key scientific research projects of colleges and universities in Henan Province(Grant No.20A550014) 

主　　题：m^(6)A methylation Chilling injury Plant hormone Nanopore direct RNA sequencing Tomato fruit 

摘      要：Tomato fruit are sensitive to chilling injury(CI)during cold *** factors have been discovered to be involved in chilling injury of tomato *** hormones play an important regulatory role,however,the relationship between chilling injury and N6-methyladenosine(m^(6)A)methylation of transcripts in plant hormone pathways has not been reported *** order to clarify the complex regulatory mechanism of m^(6)A methylation on chilling injury in tomato fruit,Nanopore direct RNA sequencing was employed.A large number of enzymes and transcription factors were found to be involved in the regulation process of fruit chilling injury,which were associated with plant hormone,such as 1-aminocyclopropane 1-carboxylate synthase(ACS),aspartate aminotransferase(AST),auxin response factor(ARF2),ethylene response factor 2(ERF2),gibberellin 20-oxidase-3(GA20ox)and jasmonic acid(JA).By conjoint analysis of the differential expression transcripts related to chilling injury andm^(6)Amethylation differential expression transcripts 41 differential expression transcripts were identified involved in chilling injury including 1-aminocyclopropane-1-carboxylate oxidase(ACO)and pectinesterase(PE)were down-regulated and heat shock cognate 70 kD protein 2(cpHSC70),HSP70-binding protein(HspBP)and salicylic acid-binding protein 2(SABP2)were *** results will provide a deeper understanding for chilling injury regulatory mechanism and post-harvest cold storage of tomato fruit.