In vivo immunostimulatory effect of the amoebocyte lysate and plasma of Asian horseshoe crab,Tachypleus gigas in a piscine model
作者机构：Department of BiotechnologyMaharaja Sriram Chandra Bhanja Deo University(Erstwhile North Orissa University)Odisha757003India ICAR-Indian Veterinary Research InstituteEastern Regional StationKolkata700037India Fish Health Management DivisionCentral Institute of Freshwater AquacultureBhubaneswar751002India
出 版 物：《Marine Life Science & Technology》 (海洋生命科学与技术（英文）)
年 卷 期：2021年第3卷第3期
页 面：355-362页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学]
主 题：Amoebocyte lysate Immunostimulatory Labeo rohita Plasma Tachypleus gigas
摘 要：Antimicrobial proteins/peptides are becoming a new generation of immunostimulants for prevention and disease control in human and animals,including aquatic *** the haemolymph of horseshoe crabs(Tachypleus)contains broad ranges of bioactive compounds,we have explored the in vivo immunostimulating potential of amoebocyte lysate and plasma using a fish *** major carp,Labeo rohita,yearlings were injected intraperitoneally with two doses of lysate and plasma at 50 and 100µg protein per *** abnormalities and/or mortalities were recorded in any *** injected with 50µg lysate and 100µg plasma protein showed significant enhancement(P0.01)in any *** the contrary,a significantly high hemolysin titre was recorded in fish that received 100µg plasma *** challenge with Aeromonas hydrophila,both lysate and plasma protein(s)cross protected the fish after 30 *** highest survival(50%)was recorded in group injected with 50µg lysate protein,followed by 45%in both 100µg lysate and plasma protein injected groups.
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