Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks

作     者：Kambulu Lukusa John Kabuba Kambulu Lukusa;John Kabuba

作者机构：Department of Animal and Wildlife SciencesUniversity of PretoriaSouth Africa Department of Chemical EngineeringVaal University of TechnologySouth Africa 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2021年第10卷第4期

页      面：187-194页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0905[农学-畜牧学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 0906[农学-兽医学] 0901[农学-作物学] 0902[农学-园艺学] 10[医学] 

基　　金：National Research Foundation, NRF, (86181, N00460) University of Pretoria, UP, (K Lukusa-23347971) 

主　　题：Post thaw Cryopreservation Saanen buck Selenium supplementation Semen collection method Sperm kinematic parameters Sperm motility Sperm quality 

摘      要：Objective:To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen ***:Sixteen healthy bucks were divided into two equal groups(n=8 each).The treatment group received selenium at 10-day intervals for three *** kinematic parameters,morphological parameters,mitochondrial membrane potential,plasma membrane functionality,and sperm DNA integrity were evaluated weekly pre and ***:The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method(P0.05).Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation(P0.05).Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups(P0.05).The sperm kinematic parameters such as curvilinear velocity,average path velocity,and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks(P0.05).The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups(P0.05).In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups,respectively(P0.05).Conclusions:Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.