Association of gene and protein expression and genetic polymorphism of CC chemokine ligand 4 in colorectal cancer

作     者：Levar Shamoun Kalle Landerholm Amanda Balboa Ramilo Roland E Andersson Jan Dimberg Dick Wågsäter 

作者机构：Department of Medical Cell BiologyUppsala UniversityUppsala 75123Sweden Department of Laboratory Medicine and PathologyRegion Jönköping CountyJönköping 55305Sweden Department of SurgeryRegion Jönköping CountyJönköping 55305Sweden Department of Biomedical and Clinical ScienceLinköping UniversityLinköping 58185Sweden Department of Natural Science and BiomedicineSchool of Health and WelfareJönköping UniversityJönköping 55111Sweden 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2021年第27卷第30期

页      面：5076-5087页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Medical Research Council of Southeast Sweden(FORSS)and Division of Medical Diagnostics(Futurum) Region Jönköping County Sweden 

主　　题：CC chemokine ligand 4 Gene polymorphism Gene and protein expression Chemokine Survival rate Colorectal cancer 

摘      要：BACKGROUND Leukocytes,such as T cells and macrophages,play an important role in *** chemokine ligand(CCL)4,which is produced by lymphocytes and macrophages,has been found to be expressed in the mucosa of the gastrointestinal tract and is a potent chemoattractant for various *** To examine CCL4 expression and its genetic polymorphism rs10491121 in patients with colorectal cancer(CRC)and evaluate their prognostic *** Luminex technology was used to determine CCL4 Levels in CRC tissue(n=98),compared with paired normal tissue,and in plasma from patients with CRC(n=103),compared with healthy controls(n=97).Included patients had undergone surgical resection for primary colorectal adenocarcinomas between 1996 and 2019 at the Department of Surgery,Ryhov County Hospital,Jönköping,*** transcription quantitative PCR was used to investigate the CCL4 gene expression in CRC tissue(n=101).Paired normal tissue and TaqMan single nucleotide polymorphism assays were used for the CCL4 rs10491121 polymorphism in 610 CRC patients and 409 healthy *** The CCL4 protein and messenger RNA expression levels were higher in CRC tissue than in normal paired tissue(90%,P0.001 and 45%,P0.05,respectively).CRC tissue from patients with localized disease had 2.8-fold higher protein expression levels than that from patients with disseminated *** CCL4 protein expression levels in CRC tissue were associated with a 30%lower cancer-specific survival rate in patients(P0.01).The level of plasma CCL4 was 11%higher in CRC patients than in healthy controls(P0.05)and was positively correlated(r=0.56,P0.01)with the CCL4 protein level in CRC *** analysis of CCL4 gene polymorphism rs10491121 showed a difference(P0.05)between localized disease and disseminated disease in the right colon,with a dominance of allele A in localized ***,the rate of the A allele was higher among CRC patients with mucinous cancer than among th