Investigation on the Metabolism of Salvianolic acid B, Tanshinone Ⅱ A, Ginsenoside Rg1 and Ginsenoside Rb1 from Danshen-Sanqi Herbal Pair in Zebrafish by Liquid Chromatography-Tandem Mass Spectrometry Analysis
作者机构：School of Chemistry and Chemical EngineeringChongqing UniversityChongqing 401331China School of Pharmacy and BioengineeringChongqing University of TechnologyChongqing 400054China
出 版 物：《Clinical Complementary Medicine and Pharmacology》 (临床补充医学和药理学（英文）)
年 卷 期：2021年第1卷第1期
页 面：1-8页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：Zebrafish Danshen-Sanqi herbal pair Metabolite Metabolic pathways Synergistic effect
摘 要：Background:Salvianolic acid B(SAB),tanshinone IIA(TIIA),ginsenoside Rg1(GRg1)and ginsenoside Rb1(GRb1)are the main active components of Danshen-Sanqi herbal pair(DS-SQ),which is widely used in the treatment of cardiovascular diseases in *** compatibility mechanisms of compound Chinese herbal preparation may be clarified to some degree by understanding the interactions of the active components based on their metabolic ***,zebrafish is a convenient and feasible model to study the metabolism of ***:To investigate the metabolism of SAB,TIIA,GRg1 and GRb1 in zebrafish by liquid chromatography-tandem mass spectrometry(LC-MS/MS)***:Zebrafish embryos after 48-hour hatching were divided into nine experimental *** blank group was exposed to 1 mL ultra-pure *** drug-treated groups were exposed to 1 mL solution of SAB,TIIA,GRg1,GRb1,SAB and GRg1,SAB and GRb1,TIIA and GRg1,TIIA and GRb1,*** homogenization,they were analyzed by LC-MS/*** Structures of the metabolites were determined by analyzing their corre-sponding mass spectra information and comparing the data with relevant *** to the change of the amount of metabolites,the metabolic effect between SAB,TIIA,GRg1 and GRb1 were ***:Eighteen metabolites and four parent drugs of SAB,TIIA,GRg1 and GRb1 in zebrafish were identified by LC-MS/MS,including methylation,degradation,reduction,dehydrogenation,hydroxylation,desugarization and glucuronidation ***,after being combined exposed of drugs,GRg1 and GRb1 could promote the metabolism of TIIA,while SAB and TIIA could promote the metabolism of GRg1 and GRb1 to different ***:By using LC-MS/MS analysis,zebrafish was successfully applied to study the metabolism of SAB,TIIA,GRg1 and GRb1,which is significance to explain the possible synergistic effect of components in DS-SQ through modifying the metabolism in *** study can be helpfu
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