Identification of hub genes and their novel diagnostic and prognostic significance in pancreatic adenocarcinoma

作     者：Duo Zuo Yongzi Chen Xinwei Zhang Zhuozhi Wang Wenna Jiang Fan Tang Runfen Cheng Yi Sun Lu Sun Li Ren Rui Liu Duo Zuo;Yongzi Chen;Xinwei Zhang;Zhuozhi Wang;Wenna Jiang;Fan Tang;Runfen Cheng;Yi Sun;Lu Sun;Li Ren;Rui Liu

作者机构：Department of Clinical Laboratory Department of Tumor Cell BiologyTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China School of Biomedical EngineeringTianjin Medical UniversityTianjin 300070China Department of PathologyTianjin Huanhu HospitalTianjin 300350China Department of PathologyTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China School of PharmacyTianjin Medical UniversityTianjin 300070China Department of Gastrointestinal Medical OncologyTianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2022年第19卷第7期

页      面：1029-1046页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the Natural Science Foundation of Tianjin City in China(Grant Nos.18JCYBJC25400 and 18JCZDJC32600)and the National Natural Science Foundation of China(Grant No.81802432) 

主　　题：RSAD2 SMC4 DLGAP5 ISG15 pancreatic adenocarcinoma 

摘      要：Objective:The main reasons for the poor prognoses of pancreatic adenocarcinoma(PA)patients are rapid early-stage progression,advanced stage metastasis,and chemotherapy *** of novel diagnostic and prognostic biomarkers of PA is therefore urgently ***:Three mRNA microarray datasets were obtained from the Gene Expression Omnibus database to select differentially expressed genes(DEGs).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses for hub genes were performed using *** between expression levels of hub genes and cancer-infiltrating immune cells were investigated by *** proportional hazard regression analyses were also *** hub genes were screened using the HPA platform and verified for diagnostic value using ***:We identified 59 hub genes among 752 *** analysis indicated that these 59 hub genes were mainly involved in the defense response to viruses and the type I interferon signaling *** also discovered that RSAD2 and SMC4 were associated with immune cell infiltration in the PA ***,DLGAP5 mRNA might be used as an independent risk factor for the prognoses of PA ***,the protein encoded by ISG15,which exists in peripheral blood,was validated as a potential diagnostic biomarker that distinguished PA patients from healthy controls(area under the curve:0.902,95%confidence interval:0.819–0.961).Conclusions:Our study suggested that RSAD2 and SMC4 were associated with immune cell infiltration in the PA microenvironment,while DLGAP5 mRNA expression might be an independent risk factor for the survival prognoses of PA ***,ELISAs indicated that serum ISG15 could be a potential novel diagnostic biomarker for PA.