Aging is associated with cardiac autonomic nerve fiber depletion and reduced cardiac and circulating BDNF levels

作     者：Andrea Elia Alessandro Cannavo Giuseppina Gambino Maria Cimini Nicola Ferrara Raj Kishore Nazareno Paolocci Giuseppe Rengo Andrea Elia;Alessandro Cannavo;Giuseppina Gambino;Maria Cimini;Nicola Ferrara;Raj Kishore;Nazareno Paolocci;Giuseppe Rengo

作者机构：Department of Translational Medical SciencesFederico Ⅱ University of Naples Italy Istituti Clinici Scientifici ICS-MaugeriTelese Terme(BN)Italy Center for Translational MedicineDepartment of PharmacologyLewis Katz School of MedicineTemple UniversityPhiladelphiaPennsylvaniaUSA Division of CardiologyJohns Hopkins University Medical InstitutionsBaltimoreMDUSA Department of Biomedical SciencesUniversity of PadovaItaly 

出 版 物：《Journal of Geriatric Cardiology》 (老年心脏病学杂志（英文版）)

年 卷 期：2021年第18卷第7期

页      面：549-559页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 071008[理学-发育生物学] 

基　　金：supported by R01 HL136918(to N.P.) STAR 2016 program(to GR) partly supported by the Italian Ministry of Education,Universities,and Research-Rita Levi Montalcini 2016(to AC) 

主　　题：cardiac depletion fibers 

摘      要：Background Aging is a multifactorial process associated with an impairment of autonomic nervous system(ANS)*** ANS remodeling includes upregulation of expression of circulating catecholamines and depletion of cardiac autonomic nerve fibers,and it is responsible,in part,for the increased susceptibility to cardiac diseases observed in elderly *** factors,such as brain-derived neurotrophic factor(BDNF)and nerve growth factor(NGF),are involved in synaptogenesis and neurite outgrowth processes,supporting neuronal cell differentiation and ***,whether and how these factors and their downstream signaling are involved in cardiac aging remains ***,we tested whether,in the aged heart,the overall extent of autonomic fibers is reduced,owing to lower production of trophic factors such as BDNF and *** In vivo,we used young(age:3 months;n=10)and old(age:24 months;n=11)male Fisher rats,whereas,we used human neuroblastoma(SH-SY5 Y)cells in *** Compared to the young rats,old rats displayed a marked reduction in the overall ANS fiber density,affecting both sympathetic and cholinergic compartments,as indicated by dopamineβ-hydroxylase(dβh)and vesicular acetylcholine transporter(Va Ch T)immunohistochemical *** addition,a marked downregulation of GAP-43 and BDNF protein was observed in the left ventricular lysates of old rats compared to those of young ***,we did not find any significant difference in cardiac NGF levels between the young and old *** further explore the impact of aging on ANS fibers,we treated SH-SY5 Y cells in vitro with serum obtained from young and old *** from both groups induced a remarkable increase in neuronal sprouting,as evidenced by a crystal violet ***,this effect was blunted in cells cultured with old rat serum and was accompanied by a marked reduction in GAP-43 and BDNF protein *** Our data indicate that physiological aging i