Molecular cloning and expression analysis of the Rf-m candidate genes encoding pentatricopeptide repeat proteins in soybean

作     者：Dagang Wang Shengnan Chen Jiekun Li Qian Wu Guoyu Hu Zhiping Huang Dagang Wang;Shengnan Chen;Jiekun Li;Qian Wu;Guoyu Hu;Zhiping Huang

作者机构：Crop Research InstituteAnhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui ProvinceHefei230031AnhuiChina College of Resources and Environment/Root Biology CenterFujian Agriculture and Forestry UniversityFuzhou350002FujianChina 

出 版 物：《Oil Crop Science》 (中国油料作物学报(英文版）)

年 卷 期：2021年第6卷第3期

页      面：128-136页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：the National Key Research and Development Program of China(Grant No.2016YFD0101503) the Key Research and Development Program of Anhui Province(Grant No.202004a06020034) the Major Science and Technology Project of Anhui Province(Grant No.18030701178) the Program on Industrial Technology System of National Soybean(Grant No.CARS-04-PS07) 

主　　题：Soybean PPR Restorer gene Cytoplasmic male sterility Quantitative RT-PCR 

摘      要：Cytoplasmic male sterility(CMS)-restorer system is a useful tool to exploit heterosis in *** major restorer gene for the M-type CMS is known as Rf-m,located in the 162.4-kb region on chromosome *** analysis has revealed that the Rf-m locus in Glycine max consists of seven penta tricopeptide repeat(GmPPR)*** deduced amino acid sequences contain 8 to 14 PPR motifs,and a phylogenetic analysis grouped these GmPPR proteins into two PPR subfamilies:Glyma.16G161800 belongs to the PLS subfamily,and the P subfamily *** Glyma.16G161900,Glyma 16G162000,Glyma.16G162100,Glyma.16G162700,Glyma.16G162800,and Gly-ma *** phylogenetic analysis of seven GmPPR proteins and 27 other plant PPR proteins also showed that proteins in the same subfamilies cluster *** sequence analysis was conducted using the seven Rf-m candidate GmPPR genes from the sterile line W931A,the maintainer line W931B,and the restorer line WR016,the result showed that Glyma 16G161900 had higher polymorphism than the other candidate *** on real-time quantitative RT-PCR data,all seven GmPPR genes were differentially expressed but showed constitutive expression in roots,stems,leaves,and pollen ***,the expression level of Gly-ma 16G161900 in the sterile line W931 A was significantly higher in all tissues than in the restorer line *** together,these results suggest that Glyma 16G161900 is the most likely candidate for the restorer gene *** study is the first report and analysis of candidate fertility restorer(Rf)genes encoding PPR proteins in soybean.