N-carbamoylglutamate improves lipid metabolism, inflammation, and apoptosis responses in visceral adipocytes of Japanese seabass (Lateolabrax japonicus), in vivo and in vitro

作     者：Haoyan Huang Xiaoran Zhang Xiaofang Liang Xiufeng Wu Xu Gu Juan Han Min Xue Haoyan Huang;Xiaoran Zhang;Xiaofang Liang;Xiufeng Wu;Xu Gu;Juan Han;Min Xue

作者机构：National Aquafeed Safety Assessment CenterFeed Research InstituteChinese Academy of Agricultural SciencesBeijing100081China Institute of Food and Nutrition DevelopmentMinistry of Agriculture and Rural AffairsChina Feed Processing Innovation TeamFeed Research InstituteChinese Academy of Agricultural SciencesBeijing100081China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第3期

页      面：707-715页

核心收录：

学科分类：0710[理学-生物学] 090801[农学-水产养殖] 0908[农学-水产] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by the National Key R&D Program of China(2019YFD0900200,2018YFD0900400) the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2017-FRI-08) Beijing Technology System for Sturgeon and Salmonids(BAIC08-2021) National Natural Science Foundation of China(31902382) Natural Science Foundation of Beijing(6204047) 

主　　题：N-Carbamylglutamate Visceral adipose and adipocytes Lipid metabolism Inflammatory and apoptosis Lateolabrax japonicus 

摘      要：This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isolated adipocytes of Japanese seabass(Lateolabrax japonicus).A basal diet and a test diet supplemented with 720 mg/kg NCG were fed to the fish for 10 *** the growth trial,no mortality and no significant differences in growth performance were observed in fish between the 2 groups(P0.05).Plasma Arg content and mRNA level of argininosuccinate synthetase(ASS)in adipose tissue were significantly increased,which indicated that NCG inclusion promoted endogenous Arg ***,the potential effects of NCG treatment on lipid metabolism-related genes expression were studied through in vivo and in vitro *** the present study,we successfully established a primary adipocytes culture system and isolated pre-adipocytes in vitro of Japanese seabass for the first *** the results in vivo and in vitro showed that NCG treatment decreased the mRNA levels of genes related to adipogenesis(fatty acid synthase,FASN),cholesterol synthesis(3-hydroxy-3-methylglutaryl-CoA reductase,HMGCR)and fat deposition(lipoprotein lipase[LPL]and leptin),which revealed the underlying mechanism of NCG on reducing fat *** results of this study demonstrated that NCG inclusion reduced the expression of inflammatory and apoptosis cytokines markedly in vivo and in *** conclusion,NCG did exert beneficial effects on ameliorating adipo-genesis,inflammation and apoptosis via promoting Arg endogenous synthesis in Japanese seabass.