Efficacy of gecko polysaccharide on suppressed immune response induced by cyclophosphamide in mice

作     者：ZHOU Xingqin CHANG Yazhou JI Bin ZHAO Hongyu SHEN Chaoyan CHANG Renan ZHOU Xingqin;CHANG Yazhou;JI Bin;ZHAO Hongyu;SHEN Chaoyan;CHANG Renan

作者机构：Department of RadiotherapyAffiliated Hospital of Nantong UniversityNangtong 226001China School of MedicineSoutheast UniversityNanjing 210009China Department of RadiotherapyAffiliated Hospital of Nantong University20 Xisi roadNangtongJiangsu Province 226001China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2021年第41卷第4期

页      面：539-545页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Jiangsu Provincial Department of Education(Jiangsu Government Scholarship for Overseas Study(No.43)) Scientific Research Project of Nantong Health and Family Planning Commission(Role of DNA Binding Protein B Related Energy Metabolism Pathway in the Development of Stem Cells MB 2019054) Nantong Science and Technology Project(Clinical Observation of Stereotactic Radiotherapy Combined with Ntetuzumab in the Treatment of Locally Advanced Pancreatic Cancer,JCZ 19092) 

主　　题：lizards polysaccharides cyclophosphamide tumor necrosis factor-alpha immune function 

摘      要：OBJECTIVE:To evaluate the efficacy of gecko polysaccharide on the cyclophosphamide-induced suppressed immune response in ***:Polysaccharides were extracted from fresh gecko for the first time using an orthogonal method and protein was removed using Sevag reagent(chloroform∶N-butanol,5∶1,v/v).The gecko polysaccharide(GPCE)content was determined by the phenol-concentrated sulfuric acid *** immunocompromised mouse model was established by intraperitoneally injecting cyclophosphamide at 100 mg/kg into 48 *** effects of GPCE on immune regulation in mice were assessed by a thymus-spleen index,serum hemolysin levels,and the proliferation of splenic *** cell CD4+,CD8+,and the CD4+/CD8+ ratio were evaluated by flow cytometry and the tumor necrosis factor-α(TNF-α)levels were measured by ***:The optimal extraction process for gecko polysaccharide included a 1∶20 ratio of material to liquid(v/v),an extraction temperature of 60℃ and a time of 2 *** polysaccharide content of the extract was 65.74%.GPCE was analyzed by HPLC and primarily contained glucose and small amounts of mannose,rha,and *** with the model,the thymus index,the spleen index were indices for GPCE increased with dose,whereas the high and medium groups exhibited significant differences(P0.05);The high GPCE dose significantly increased the level of serum hemolysin(P0.01).CONCLUSIONS:Gecko polysaccharide significantly improved the suppressed immune response induced by cyclophosphamide in mice and promoted the secretion of tumor necrosis *** mechanism of gecko polysaccharide as an a