Zooming in to acquire micro-reaction:Application of microfluidics on soil microbiome

作     者：Xiongkun Zhang Shan Wu Xiaojie Sun Monika Mortimer Yichao Wu Ming Zhang Qiaoyun Huang Peng Cai Xiongkun Zhang;Shan Wu;Xiaojie Sun;Monika Mortimer;Yichao Wu;Ming Zhang;Qiaoyun Huang;Peng Cai

作者机构：State Key Laboratory of Agricultural MicrobiologyCollege of Resources and EnvironmentHuazhong Agricultural UniversityWuhan 430070China Institute of Environmental and Health SciencesCollege of Quality and Safety EngineeringChina Jiliang UniversityHangzhou 310018China 

出 版 物：《Soil Ecology Letters》 (土壤生态学快报（英文）)

年 卷 期：2022年第4卷第3期

页      面：213-223页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0818[工学-地质资源与地质工程] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0713[理学-生态学] 090301[农学-土壤学] 

基　　金：the National Natural Science Foundation of China(41877029,41961130383) Royal SocietyNewton Advanced Fellowship(NAF\R1\191017) Wuhan Science and Technology Bureau(2019020701011469) 

主　　题：Microfluidics Soil bacteria Biofilms Chemotaxis Quorum sensing Horizontal gene transfer 

摘      要：Microfluidics confers unique advantages in microbiological studies as these devices can accurately replicate the micro-and even nano-scale structures of soil to simulate the habitats of *** not only helps us understand the spatial distribution of bacterial communities(such as biofilms),but also provides mechanistic insights into microbial behaviors including chemotaxis and horizontal gene transfer(HGT).Microfluidics provides a feasible means for real-time,in situ studies and enables indepth exploration of the mechanisms of interactions in the soil *** review aims to introduce the basic principles of microfluidic technology and summarize the recent progress in microfluidic devices to study bacterial spatial distribution and functions,as well as biological processes,such bacterial chemotaxis,biofilm streamers(BS),quorum sensing(QS),and *** challenges in and future development of microfluidics for soil microbiological studies are also discussed.