Experimental study of anti-tumor effects of polysaccharides from Angelica sinensis

作     者：Wen-MingZhao Zhi-NanChen PengShang Ai-RongQian Tie-HongYang MinJia Qi-BingMei Chi-HinCho 

作者机构：SchoolofLifeScienceandTechnologyXi'anJiaotongUniversityXi’an710054ShaanxiProvinceChina DepartmentofCellBiologyForthMilitaryMedicalUniversity.Xi'an710032ShaanxiProvinceChina DepamnentofPharmacologyFourthMilitaryMedicalUniversityXi’an710032ShaanxiProvinceChina DepartmentofPharmacologyFacultvofMedicine.UniversityofHongKongHongKongChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第9期

页      面：1963-1967页

核心收录：

学科分类：100703[医学-生药学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：RGC grant from the University of Hong Kong and the Hong Kong Research Grant Council(HKU7257-98M) the Hi-Tech Research & Development Program of China(2001AA215061) 

主　　题：当归 中药 多糖 抗肿瘤作用 

摘      要：AIM: To investigate the in vivo anti-tumor effects of total polysaccharide (AP-0) isolated from Angelica sinensis (Oliv.)Diels (Danggui) on mice and thein vitro inhibitory effects of AP-0 and its sub-constituents (AP-1, AP-2 and AP-3) on invasion and metastasis of human hepatocellular ***: Three kinds of murine tumor models in vivo,sarcoma 180 (S180), leukemia L1210 and Ehrlich ascitic cancer (FAC) were employed to investigate the anti-tumor effects of AP-0. For each kind of tumor model, three experimental groups were respectively given AP-0 at doses of 30, 100 and 300 mg/kg byip once a day for 10 *** control groups were respectively given Cy at a dose of 30 mg/kg for S180 and leukemia L1210, and 5-FU at a dose of 20 mg/kg for EAC. On d 11, mice bearing S180were sacrificed and the masses of tumors, spleens and thymus were weighed. The average living days of mice bearing EAC and of mice bearing L1210 were observed,and the rates of life prolongation of each treatment were calculated, respectively. The inhibitory effects of APs on hepatoma invasion and metastasis in vitro were investigated by employing human hepatocellular carcinoma cell line (HHCC) with the Matrigel invasion chamber, adhesion to extracelluler matrix and chemotatic migration tests, ***: AP-0 had no obviously inhibitory effect on the growth of S180, but it could significantly decrease the thymus weights of the mice bearing S180. AP-0 could significantly reduce the production of ascitic liquids and prolong the life of mice bearing EAC. AP-0 could also increase the survival time of mice bearing L1210. AP-0 and AP-2 had significantly inhibitory effects on the invasion of HHCC into the Matrigel reconstituted basement membrane with the inhibitory rates of 56.4% and 68.3%, respectively. AP-0, AP-1, AP-2 and AP-3 could influence the adhesion of HHCC to extracellular matrix proteins (Matrigel and fibronectin) at different degrees, among them only AP-3 had significant blo