TLR7/8 signalling affects X-sperm motility via the GSK3α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

作     者：Fa Ren Huaming Xi Yijie Ren Yu Li Fei Wen Ming Xian Mengjie Zhao Dawei Zhu Liqiang Wang Anmin Lei Jianhong Hu Fa Ren;Huaming Xi;Yijie Ren;Yu Li;Fei Wen;Ming Xian;Mengjie Zhao;Dawei Zhu;Liqiang Wang;Anmin Lei;Jianhong Hu

作者机构：Key Laboratory of Animal GeneticsBreeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityNo.22 Xinong RoadYanglingShaanxi 712100People’s Republic of China College of Veterinary MedicineNorthwest A&F UniversityYangling 712100ShaanxiChina 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第2期

页      面：401-417页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：This research was supported by the National Natural Science Foundation of China(31672425) Shaanxi Province Key R&D Program(2018ZDXM-NY-043,2020ZDLNY02–04) 

主　　题：Dairy goat Glycogen synthase kinaseα/β(GSK3α/β) Sexing control Sperm Toll-like receptor 7/8(TLR7/8) 

摘      要：Background:Goat milk is very similar to human milk in terms of its abundant nutrients and ease of *** derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspring sex ratio is approximately 50%.At present,artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock ***,flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy ***:In this study,a novel,simple goat sperm sexing technology that activates the toll-like receptor 7/8(TLR7/8),thereby inhibiting X-sperm motility,was *** results showed that the TLR7/8 coding goat Xchromosome was expressed in approximately 50%of round spermatids in the testis and sperm,as measured from cross-sections of the epididymis and ejaculate,***,TLR7/8 was located at the tail of the *** TLR7/8 activation,phosphorylated forms of glycogen synthase kinaseα/β(GSK3α/β)and nuclear factor kappa-B(NF-κB)were detected in the X-sperm,causing reduced mitochondrial activity,ATP levels,and sperm ***-motility Y-sperm segregated to the upper layer and the low-motility X-sperm,to the lower *** in vitro fertilisation using the TLR7/8-activated sperm from the lower layer,80.52±6.75%of the embryos were XX *** TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response;nine embryos were collected from the uterus of two does that *** of these were XX embryos,and one was an XY ***:Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3α/β-hexokinase pathway;this technique could be used to facilitate the efficient production of sexed dairy goat embryos.