Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis

作     者：Jing-Hua Shi Li Jin Jin-Hua Leng Jing-He Lang Shi Jing-Hua;Jin Li;Leng Jin-Hua;Lang Jing-He

作者机构：Department of Obstetrics and Gynecology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100730 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2016年第129卷第2期

页      面：200-205页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This study was supported by a grant of National Natural Science Foundation of China 

主　　题：Adenomyosis Potassium Channels Uterine Contraction Uterus Smooth Muscle Cells 

摘      要：Background： Adenomyosis （AM） has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells （MSMCs） of AM. Methods： Uterine tissue samples from 22 patients （cases） with histologically confirmed AM and 12 （controls） with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calciumand voltage-sensitive K＋ channel （BKCa）-a/β subunits, voltage-gated potassium channel （Kv） 4.2, and Kv4.3. Student＇s t-test was used to compare the expression. Results： The BKCa-a/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-a messenger RNA （mRNA） （0.62 ± 0.19-fold decrease, P 〈 0.05） and Kv4.3 mRNA （0.67 ±0.20-fold decrease, P 〈 0.05） decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA. Conclusions： The BKCa-a mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.