Flexible,video-rate,and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing

作     者：RUI JIN YALAN YU DAN SHEN QINGMING LUO HUI GONG JING YUAN 

作者机构：Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China Current address:Department of Mechanical and Automation EngineeringChinese University of Hong KongShatinHong Kong HUST-Suzhou Institute for BrainsmaticsJITRI Institute for BrainsmaticsSuzhou 215123China School of Biomedical EngineeringHainan UniversityHaikou 570228China 

出 版 物：《Photonics Research》 (光子学研究（英文版）)

年 卷 期：2021年第9卷第8期

页      面：1477-1485页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 080203[工学-机械设计及理论] 0802[工学-机械工程] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：National Natural Science Foundation of China(61890950,61890953,91749209) Fundamental Research Funds for the Central Universities(2019kfy XMBZ039) 

主　　题：axial field aberration 

摘      要：Parallel dual-plane imaging with a large axial interval enables the simultaneous observation of biological structures and activities in different views of ***,the inflexibility in adjusting the field-of-view(FOV)positions in three dimensions and optical sectioning effects,as well as the relatively small effective axial range limited by spherical aberration,have hindered the application of parallel dual-plane ***,we propose a flexible,video-rate,and defocus-aberration-compensated axial dual-line scanning imaging *** used a stepped mirror to remotely generate and detect dual axial lines with compensation for spherical aberration and FOV-jointing to rearrange into a head-to-head line for high-speed optical sectioning *** lateral and axial positions of the two FOVs could be flexibly adjusted before and during imaging,*** method also allows the adjustment of optical sectioning effects according to specific experimental *** experimentally verified the consistent imaging performance over an axial range of 300μ*** demonstrated high throughput by simultaneously imaging Brownian motions in two 250μm×250μm FOVs with axial and lateral intervals of 150μm and 240μm,respectively,at 24.5 *** also showed potential application in functional imaging by simultaneously acquiring neural activities in the optic tectum and hindbrain of a zebrafish *** proposed method is,thus,advantageous compared to existing parallel dual-plane imaging and potentially facilitates intravital biological study in large axial range.