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Flexible,video-rate,and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing

Flexible, video-rate, and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing

作     者:RUI JIN YALAN YU DAN SHEN QINGMING LUO HUI GONG JING YUAN 

作者机构:Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China Current address:Department of Mechanical and Automation EngineeringChinese University of Hong KongShatinHong Kong HUST-Suzhou Institute for BrainsmaticsJITRI Institute for BrainsmaticsSuzhou 215123China School of Biomedical EngineeringHainan UniversityHaikou 570228China 

出 版 物:《Photonics Research》 (光子学研究(英文版))

年 卷 期:2021年第9卷第8期

页      面:1477-1485页

核心收录:

学科分类:070207[理学-光学] 07[理学] 08[工学] 080203[工学-机械设计及理论] 0802[工学-机械工程] 0803[工学-光学工程] 0702[理学-物理学] 

基  金:National Natural Science Foundation of China(61890950,61890953,91749209) Fundamental Research Funds for the Central Universities(2019kfy XMBZ039) 

主  题:axial field aberration 

摘      要:Parallel dual-plane imaging with a large axial interval enables the simultaneous observation of biological structures and activities in different views of ***,the inflexibility in adjusting the field-of-view(FOV)positions in three dimensions and optical sectioning effects,as well as the relatively small effective axial range limited by spherical aberration,have hindered the application of parallel dual-plane ***,we propose a flexible,video-rate,and defocus-aberration-compensated axial dual-line scanning imaging *** used a stepped mirror to remotely generate and detect dual axial lines with compensation for spherical aberration and FOV-jointing to rearrange into a head-to-head line for high-speed optical sectioning *** lateral and axial positions of the two FOVs could be flexibly adjusted before and during imaging,*** method also allows the adjustment of optical sectioning effects according to specific experimental *** experimentally verified the consistent imaging performance over an axial range of 300μ*** demonstrated high throughput by simultaneously imaging Brownian motions in two 250μm×250μm FOVs with axial and lateral intervals of 150μm and 240μm,respectively,at 24.5 *** also showed potential application in functional imaging by simultaneously acquiring neural activities in the optic tectum and hindbrain of a zebrafish *** proposed method is,thus,advantageous compared to existing parallel dual-plane imaging and potentially facilitates intravital biological study in large axial range.

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