Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line
Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line作者机构:Henan Key Laboratory of Chinese Medicine for Respiratory DiseaseHenan University of Chinese MedicineZhengzhou 450046China Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of ChinaHenan University of Chinese MedicineZhengzhou 450046China
出 版 物:《Journal of Traditional Chinese Medicine》 (中医杂志(英文版))
年 卷 期:2021年第41卷第3期
页 面:360-366页
核心收录:
学科分类:1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 10[医学]
基 金:Supported by National Natural Science Fund of China (No.81130062, 81403367) the National Key Technology R&D Program during the 12th Five-Year Plan Period(2014BAI10B06)。
主 题:cigarette smoking janus kinase 2 STAT3 transcription factor THP-1 cells matrix metalloproteinases Tiaobu Feishen therapy
摘 要:OBJECTIVE: To study the mechanistic effects of Tiaobu Feishen therapy(TBFS) on inflammation induced by cigarette smoke extract(CSE) in a human monocyte/macrophage cell line.METHODS: The human monocyte/macrophage cell line THP-1 was stimulated with 10% CSE in the presence or absence of Bufei Yishen formula(BYF),Bufei Jianpi formula(BJF) and Yiqi Zishen formula(YZF). All formulations contained serum. Pro-inflammatory cytokines were measured in the supernatants using enzyme-linked immunosorbent assay.The activity of STAT3 DNA binding was detected using electrophoretic mobility shift assay and janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway activation was assessed using Western blotting.RESULTS: The results showed that BYF, BJF and YZF treatment strongly decreased the CSE-induced secretion of interleukin(IL)-6, IL-8, tumor necrosis factor-α and matrix metalloproteinase-9 by THP-1 cells. Furthermore, BYF, BJF and YZF treatment attenuated STAT3 DNA binding capacity and JAK2 and STAT3 were shown to be phosphorylated.CONCLUSION: The data revealed that BYF, BJF and YZF effectively inhibited a CSE-induced inflammatory response in THP-1 cells by limiting activation of the JAK2/STAT3 pathway.