Rapid diagnosis of disseminated Mycobacterium mucogenicum infection in formalin-fixed, paraffin-embedded specimen using nextgeneration sequencing: A case report
作者机构：Department of Infectious DiseasesThe Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510630Guangdong ProvinceChina Department of EmergencyThe First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina Department of PharmacyThe Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510630Guangdong ProvinceChina State Key Laboratory of Respiratory DiseaseThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120Guangdong ProvinceChina
出 版 物：《World Journal of Clinical Cases》 (世界临床病例杂志)
年 卷 期：2021年第9卷第20期
页 面：5621-5630页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：Supported by the Clinical Research Foundation of the Third Affiliated Hospital of Sun Yat-Sen University,No.YHJH201904 National Science and Technology Major Project,No.2018ZX10302204
主 题：Mycobacterium mucogenicum Next-generation sequencing Disseminated infection Formalin-fixed,paraffin-embedded specimen Rapid diagnosis Case report
摘 要：BACKGROUND Mycobacterium mucogenicum(***)belongs to the group of rapidly growing Nontuberculous *** microorganism is associated with a wide spectrum of infectious *** to a low detection rate or the time required for conventional culture methodology,a rapid and broad-spectrum method is necessary to identify rare *** SUMMARY A 12-year-old immunocompetent girl presented with painful masses for five *** first mass was found in the right upper quadrant of the abdomen,and was about 1 cm×1.5 cm in size,tough but pliable in texture,with an irregular margin and *** abscess gradually formed and ulcerated with suppuration of the *** new masses appeared on the back one by *** computed tomography showed patchy and streaky cloudy opacities in both *** aspiration of the abscess was performed,but the smear and conventional culture were negative,and the pathological examination showed no *** then performed next-generation sequencing using a formalinfixed,paraffin-embedded specimen to identify the pathogen.A significantly high abundance of *** was *** patient’s abscesses gradually decreased in size,while inflammation in both lungs improved following 12-wk of *** recurrence was observed four months after the end of the one-year treatment *** Next-generation sequencing is a promising tool for the rapid and accurate diagnosis of rare pathogens,even when using a formalin-fixed,paraffin-embedded specimen.
维普期刊数据库