Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus(krait) venom

作     者：Adil Shah Mushtaq Ahmed Naila Sher Nadia Mushtaq Rahmat Ali Khan Fozia Midrarullah Adil Shah;Mushtaq Ahmed;Naila Sher;Nadia Mushtaq;Rahmat Ali Khan;Fozia;Midrarullah

作者机构：Department of BiotechnologyFaculty of Biological SciencesUniversity of Science&TechnologyBannu 28100KPK-Pakistan Department of BotanyFaculty of Biological SciencesUniversity of Science&TechnologyBannu 28100KPK-Pakistan Department of BiochemistryKMU Institute of Medical SciencesKohat 26000Pakistan Department of BiotechnologySaheed Benazir Bhutto UniversitySheringalDir UpperKPK-Pakistan 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2021年第41卷第3期

页      面：349-354页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by Higher Education Commission of Pakistan(HEC-PAK No.20-2071/NRPU) Directorate of Science and Technology (DoST) Khyber Pakhtunkhwa。 

主　　题：acetylcholinesterase snake venoms silene arenosa competitive inhibition 

摘      要：OBJECTIVE:To examine the efficacy of Silene arenosa extract on acetylcholinesterase (AChE) of krait (Bungarus Sindanus) snake venom.METHODS:The present project designed to evaluate the inhibition of AChE by following standard procedures.RESULTS:Statistical analysis of the results showed that Silene arenosa exerted 73%inhibition against the krait venom acetylcholinesterase at fixed substrate acetylcholine (ACh) concentration (0.5 mM).Kinetic analysis using the Lineweaver Burk plot revealed that Silene arenosa caused a competitive type of inhibition i.e.K_(m) values increased from 26.6 to 93.3 mM (26.6%to 93.3%) and V_(max)remained constant in a concentration-dependent manner.Silene arenosa competes with the substrate to bind at the active site of the enzyme.The K_(mapp)of venom AChE for Silene arenosa increased from 60%to 81.6%and the V_(maxapp)remains constant.K_(i)(inhibition constant was estimated to be 48μg for snake venom);while the K_(m)(Michaelis-Menten constant of AChE-substrate into AChE and product) was estimated to be 0.5 mM.The IC_(50)of AchE calculated for Silene arenosa was 67μg.CONCLUSION:The present results suggest that Silene arenosa extract can be considered as an inhibitor of snake venom AChE.