Intravitreal brimonidine inhibits formdeprivation myopia in guinea pigs

作     者：Yifang Yang Junshu Wu Defu Wu Qi Wei Tan Zhong Jun Yang Xiaowei Yang Meizhen Zeng Xingwu Zhong Yifang Yang;Junshu Wu;Defu Wu;Qi Wei;Tan Zhong;Jun Yang;Xiaowei Yang;Meizhen Zeng;Xingwu Zhong

作者机构：State Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Hainan Eye Hospital and Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen UniversityHaikou 570311Hainan ProvinceChina 

出 版 物：《Eye and Vision》 (眼视光学杂志（英文）)

年 卷 期：2021年第8卷第5期

页      面：263-275页

核心收录：

学科分类：1204[管理学-公共管理] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.81870681) the Natural Science Foundation of Hainan Province(Grant No.817364) 

主　　题：Brimonidine Myopia Form deprivation Retina 

摘      要：Background:The use of ocular hypotensive drugs has been reported to attenuate myopia *** study explores whether brimonidine can slow myopia progression in the guinea pig form-deprivation(FD)***:Three-week-old pigmented male guinea pigs(Cavia porcellus)underwent monocular FD and were treated with 3 different methods of brimonidine administration(eye drops,subconjunctival or intravitreal injections).Four different concentrations of brimonidine were tested for intravitreal injection(2μg/μL,4μg/μL,20μg/μL,40μg/μL).All treatments continued for a period of 21 ***,retinoscopy,and A-scan ultrasonography were used to monitor intraocular pressure(IOP),refractive error and axial length(AL),*** day 21,guinea pigs were sacrificed for RNA sequencing(RNA-seq)to screen for associated transcriptomic ***:The myopia model was successfully established in FD animals(control eye *** eye,respectively:refraction at day 20,0.97±0.18 D vs.−0.13±0.38 D,F=6.921,P=0.02;AL difference between day 0 and day 21,0.29±0.04mm vs.0.45±0.03 mm,F=11.655,P=0.004).Among the 3 different brimonidine administration methods,intravitreal injection was the most effective in slowing myopia progression,and 4μg/μL was the most effective among the four different concentrations of brimonidine intravitreal injection *** AL and the refraction of the brimonidine intravitreal injection group was significantly shorter or more hyperopic than those of other 2 ***μg/μL produced the smallest difference in AL and spherical equivalent difference *** treatment significantly increased the *** was significantly lower at 1 day after intravitreal injections which was the lowest in FD eye of intravitreal injection of *** day 21,gene expression analyses using RNA-seq showed upregulation of Col1a1 and Mmp2 expression levels by intravitreal ***:Among the 3 different administration methods,intravitreal injection of brimonid