Aneuploidy:An opportunity within single-cell RNA sequencing analysis

作     者：JOE R.DELANEY 

作者机构：Department of Biochemistry and Molecular BiologyMedical University of South CarolinaCharleston29425USA 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2021年第45卷第5期

页      面：1167-1170页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：NIH NCI Grant[CA207729](JD) 

主　　题：scRNA-seq Copy-number alterations Aneuploidy Cancer 

摘      要：Single-cell sequencing data has transformed the understanding of biological *** many flavors of single-cell sequencing have been developed,single-cell RNA sequencing(scRNA-seq)is currently the most prolific form in published *** analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene *** biologically relevant variable is readily inferred from scRNA-seq gene count tables regardless of individual gene representation within single cells:*** hundreds of genes are present on chromosome arms,high-quality inferences of aneuploidy can be made from scRNA-seq *** viewpoint summarizes how utilization of these bioinformatic pipelines can benefit scRNA-seq studies,particularly in oncology wherein aneuploidy is both rampant and a hallmark of the studied *** and use of these analytical pipelines will improve each field’s ability to understand the studied *** are encouraged to attempt these aneuploid analyses when reporting scRNA-seq data,much like copy-number variants are commonly reported in bulk genome sequencing data.