Expression and role of P-element-induced wimpy testis-interacting RNA in diabetic-retinopathy in mice
作者机构：Department of OphthalmologyShengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina Department of Thoracic SurgeryShengjing Hospital of China Medical UniversityShenyang 110004Liaoning ProvinceChina
出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)
年 卷 期：2021年第12卷第7期
页 面：1116-1130页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by National Natural Science Foundation of China No.81570866
主 题：P-element-induced wimpy testis-interacting RNA P-element-induced wimpy testis protein High-throughput sequencing Neovascularization Diabetic retinopathy in mice Bioinformatics
摘 要：BACKGROUND As one of the major microvascular complications of diabetes,diabetic retinopathy(DR)is the leading cause of blindness in the working age *** the extremely complex pathogenesis of DR has not been fully clarified,the occurrence and development of DR is closely related to tissue ischemia and hypoxia and neovascularization The formation of retinal neovascularization(RNV)has great harm to the visual acuity of *** To investigate the expression of P-element-induced wimpy testis-interacting RNA(piRNA)in proliferative DR mice and select piRNA related to *** One hundred healthy C57BL/6J mice were randomly divided into a normal group as control group(CG)and proliferative DR(PDR)group as experimental group(EG),with 50 mice in each *** were collected from both groups at the same time,and the lesions of mice were evaluated by hematoxylin and eosin staining and retinal blood vessel *** retinal tissues were collected for second-generation high-throughput sequencing,and the differentially expressed piRNA between the CG and EG was detected,and polymerase chain reaction(PCR)was conducted for *** differentially obtained piRNA target genes and expression profiles were enrichment analysis based on gene annotation(Gene Ontology)and Kyoto Encyclopedia of Genes and *** In the CG there was no perfusion area,neovascularization and endothelial nucleus broke through the inner boundary membrane of *** the EG,there were a lot of nonperfused areas,new blood vessels and endothelial nuclei breaking through the inner boundary membrane of the *** was a statistically significant difference in the number of vascular endothelial nuclei breaking through the inner retinal membrane between the two ***-throughput sequencing analysis showed that compared with the CG,a total of 79 piRNAs were differentially expressed in EG,among which 43 piRNAs were up-regulated and 36 piRNAs were ***
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