Novel pneumatically assisted atomization device for living cell delivery:application of sprayed mesenchymal stem cells for skin regeneration

作     者：Lixing Zhang Xintao Yan Li An Meijia Wang Xi Xu Zhonglin Ma Mengting Nie Fangzhou Du Jingzhong Zhang Shuang Yu Lixing Zhang;Xintao Yan;Li An;Meijia Wang;Xi Xu;Zhonglin Ma;Mengting Nie;Fangzhou Du;Jingzhong Zhang;Shuang Yu

作者机构：Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163China Zhengzhou Institute of Engineering and Technology Affiliated with SIBETZhengzhou 450001China Xuzhou Medical UniversityXuzhou 221004China Academy for Engineering and TechnologyFudan UniversityShanghai 200433China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2022年第5卷第1期

页      面：220-232页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 08[工学] 10[医学] 

基　　金：the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020807) the Key Research and Development Program of Jiangsu Province,China(Nos.BE2018668 and BE2017669) the Major Innovative Research Team of Suzhou,China(No.ZXT2019007) SIBET and Jilin City Science and Technology Cooperation Project(No.E0550104) Doctor of Entrepreneurship and Innovation Program of Jiangsu Province in the year of 2020 

主　　题：Cell delivery Skin wound Wound healing Spray application Cell-spray autografting Stem cell therapy 

摘      要：Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is ***,topical stem cell therapy has been realized as a promising strategy for wound healing and skin ***,stem cells must be administrated uniformly to the wound area,otherwise treatment will be ineffective,which has been a limitation of current administration ***,the delivery pressure and nozzle features of most clinical cell spray devices are unknown,which may significantly affect the viability of sprayed cells and their capacity for ***,we developed a novel pneumatically assisted atomization device(PAAD)in which cell suspensions were uniformly atomized at a low delivery *** optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types,while ensuring uniform dispersal across the wound ***,we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound ***,the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells,strongly suggesting its potential for application in clinical cell therapy,especially for large,irregular,and severe skin wounds.