Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro
作者机构：Laboratorio de Inmuno-RegulaciónImmunology DepartmentPabellón de Medicina y Cirugía ExperimentalHospital General Universitario Gregorio MarañónInstituto de Investigación Sanitaria Gregorio Marañón(IiSGM)Calle Máiquez9Madrid 28009Spain Laboratorio de Inmuno-Biología MolecularImmunology DepartmentHospital General Universitario Gregorio MarañónInstituto de Investigación Sanitaria Gregorio Marañón(IiSGM)C/Dr.Esquerdo 46Madrid 28007Spain Networking Research Center on BioengineeringBiomaterials and Nanomedicine(CIBER-BBN)C/Monforte de Lemos 3-5Pabellón 11Madrid 28029Spain
出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)
年 卷 期：2018年第15卷第10期
页 面：917-933页
核心收录：
学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：This work was partially supported by Ministry of Economy and Competitiveness ISCIII-FIS grants PI12/01763,PI12/00934 and PI15/00923 co-financed by ERDF(FEDER)Funds from the European Commission,‘A way of making Europe’ JL-A was supported by a Grant from IiSGM AP-S was supported by the Youth Employment Program co-financed by the Madrid community and FEDER Founds RC-R was supported by the‘Miguel Servet’program(CPII13/00033) MP by the Spanish MICINN through the Ramón y Cajal(RYC-2009-05486).
主 题：HIV-1 immune hyper-activation regulatory B cells
摘 要：Individuals infected with human immunodeficiency virus type-1(HIV-1)usually show a general dysregulation and hyper-activation of the immune system.A direct influence of HIV-1 particles on B-cell phenotypes and functions has been previously described.However,the consequences of B-cell dysregulation are still poorly understood.We evaluated the phenotypic changes in primary B cells after direct contact with HIV-1 particles in comparison with different types of stimuli.The functionality of treated B cells was challenged in co-culture experiments with autologous CD4+and CD8+T cells.We demonstrated that HIV-1 induces a phenotypic change in B cells towards a regulatory B-cell phenotype,showing a higher level of IL-10,TGF-β1,EBI3 or IL-12(p35)mRNA expression and acquiring an immunosuppressive profile.The acquisition of a Breg phenotype was confirmed by co-culture experiments where HIVtreated B cells reduced the proliferation and the TNFαproduction of CD4+or CD8+T cells.This suppressive ability of HIV-treated B cells was dependent on cell-to-cell contact between these B cells and effector cells.To our knowledge,these data provide the first evidence that HIV-1 can directly induce a regulatory B cell-like immunosuppressive phenotype,which could have the ability to impair specific immune responses.This dysregulation could constitute one of the mechanisms underlying unsuccessful efforts to develop an efficient vaccine against HIV-1.
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