A protocol to develop T helper and Treg cells in vivo

作     者：Weiqian Chen Zhenjian Xu Yongjiang Zheng Julie Wang Wenbin Qian Nancy Olsen David Brand Jin Lin Song Guo Zheng 

作者机构：Division of RheumatologyFirst Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang Province 310003China Division of RheumatologyDepartment of MedicinePenn State University Hershey College of Medicine HersheyHersheyPA 17033USA Department of Clinical ImmunologyThird Affiliated HospitalSun Yat-sen UniversityGuangzhou 510120China Division of HematologyFirst Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang Province 310003China Research ServiceMemphis VA Medical CenterMemphisTNUSA 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2017年第14卷第12期

页      面：1013-1016页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

基　　金：part by grants from the National Key R&D Program of China(2017YFA0105800) NIH R01 AR059103,NIH STAR award,NIH R61 AR073049 National Natural Science Foundation of China(81671611,81701600) Guangdong Province Innovative Research Program Project(2011Y035) Zhejiang Provincial Natural Science Foundation of China(LY14H100002) 

主　　题：Th17 vivo Treg 

摘      要：An understanding of the development and function of T helper(Th)1,Th17 and regulatory T(Treg)cells is critical toward revealing pro-inflammatory immune responses,especially in autoimmune ***,there is no standard protocol to monitor the development of these cells over time in *** protocol details a method to generate Th1,Th17,and Treg cells in a T cell-induced colitis model in vivo and monitor their dynamic changes,which can be used for further investigations in their development from naive CD4+T cells in specific gene knockouts and background strains.