Exploring the role of autophagy during early human embryonic development through single-cell transcriptome and methylome analyses

作     者：Shi Song Qianying Guo Yiru Zhu Peng Yuan Zhiqiang Yan Liying Yan Jie Qiao Shi Song;Qianying Guo;Yiru Zhu;Peng Yuan;Zhiqiang Yan;Liying Yan;Jie Qiao

作者机构：Center for Reproductive MedicineDepartment of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital)Beijing 100191China Key Laboratory of Assisted Reproduction(Peking University)Ministry of EducationBeijing 100191China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive TechnologyBeijing 100191China Beijing Advanced Innovation Center for GenomicsBeijing 100871China Peking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation ArrestChinese Academy of Medical SciencesBeijing 100191China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第5期

页      面：940-952页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

基　　金：supported by the National Key Research and Development Program(2019YFA0801400,2017YFA0103801 2018YFC1004000) the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020703) 

主　　题：autophagy single-cell RNA-seq methylation early human embryo 

摘      要：Early human embryogenesis is a very sophisticated process due to its unique gene regulatory *** has been suggested to play an important role in mediating the development of early embryonic cells in ***,evidence showing how autophagy regulates early human embryogenesis remains to be further *** this study,we systematically investigated the human transcriptome and methylome patterns of autophagy-related(ATG)genes in early embryonic cells at single-cell *** analyzed the transcriptomic data of 365 cells and methylome data of 265 *** results showed that most ATG genes remained epigenetically active and were expressed stably throughout early embryogenesis,whereas the dynamics varied among different developmental *** evidence indicated that the autophagy pathway was constitutively activated and exerted a fundamental role in early human embryo *** work,for the first time,comprehensively reveals the features of autophagy during early human embryo development.