Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method

作     者：Roujian Lu Peihua Niu Li Zhao Huijuan Wang Wenling Wang Wenjie Tan 

作者机构：Key Laboratory of BiosafetyNational Health and Family Planning CommissionNational Institute for Viral Disease Control and PreventionChina CDCBeijingChina 

出 版 物：《China CDC weekly》 (中国疾病预防控制中心周报（英文）)

年 卷 期：2020年第2卷第25期

页      面：447-452页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：This work was supported by the National Key Research and Development Program of China(2016YFD0500301) 

主　　题：Sanger clinical acute 

摘      要：What is already known on this topic?Coronavirus disease 2019(COVID-19),a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)or COVID-19 virus,was reported in December *** genomes of the COVID-19 virus from clinical samples using next generation sequencing(NGS)have been *** is added by this report?Here we provide the technical data for sequencing complete genome of COVID-19 virus from clinical samples using the Sanger *** complete COVID-19 virus genome sequences(named WH19004-S and GX0002)were obtained from clinical samples of COVID-19 patients,and two single nucleotide polymorphisms(SNPs)in ORF7a(T/C,nt 27,493)and ORF8(T/C,nt 28,253)of WH19004-S were identified by Sanger *** are the implications for public health practice?The COVID-19 virus genome sequencing by Sanger method reported here could be used to generate data of high enough quality without requirement for expensive NGS equipment,which support sequencing complete genomes from clinical samples and monitoring of viral genetic variations of COVID-19 infections.