Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

作     者：Bei Qian Jialun Li Ke Guo Nengqiang Guo Aimei Zhong Jie Yang Jiecong Wang Peng Xiao Jiaming Sun Lingyun Xiong 

作者机构：Department of Plastic SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology1277 Jiefang AvenueWuhan 430022China Wuhan Clinical Research Center for Superficial Organ ReconstructionWuhan 430022China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第2期

页      面：74-84页

核心收录：

学科分类：1002[医学-临床医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：This study received financial support by the National Key R&D Program of China(2019YFA0110500) the National Natural Science Foundation of China(82020108020,81873941 and 81701922). 

主　　题：collagen graphene oxide N-acetylcysteine sustained release diabetic wound healing 

摘      要：Associated with persistent oxidative stress,altered inflammatory responses,poor angiogenesis and epithelization,wound healing in diabetic patients is impaired.N-acetylcysteine(NAC)is reported to resist excess reactive oxygen species(ROS)production,prompt angiogenesis and maturation of the epidermis.Studies have revealed that graphene oxide(GO)can regulate cellular behavior and form cross-links with naturally biodegradable polymers such as collagen(COL)to construct composite scaffolds.Here,we reported a COL-based implantable scaffold containing a mixture of GO capable of the sustained delivery of NAC to evaluate the wound healing in diabetic rats.The morphological,physical characteristics,biocompatibility and NAC release profile of the GO-COL-NAC(GCN)scaffold were evaluated in vitro.Wound healing studies were performed on a 20mm dorsal full-skin defect of streptozotocin(STZ)-induced diabetic rats.The injured skin tissue was removed at the 18th day post-surgery for histological analysis and determination of glutathione peroxidase(GPx),catalase(CAT)and superoxide dismutase(SOD)activity.In diabetic rats,we confirmed that the GCN scaffold presented a beneficial effect in enhancing the wound healing process.Additionally,due to the sustained release of NAC,the scaffold may potentially induce the antioxidant defense system,upregulating the expression levels of the antioxidant enzymes in the wound tissue.The findings revealed that the antioxidant biocompatible composite collagen dressing could not only deliver NAC in situ for ROS inhibition but also promote the wound healing process.This scaffold with valuable therapy potential might enrich the approaches for surgeon in diabetic wound treatment in the future.