Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice

作     者：Jing-hua Liu Li-ping DOU Li-xin Wang Li-li Wang Fan Zhou Li Yu 

作者机构：Department of Hematology Shenyang Military General Hospital Shenyang 110016 China Department of Hematology General Hospital of the Chinese People＇s Liberation Army Beijing 100853 China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2011年第26卷第2期

页      面：91-97页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 081302[工学-建筑设计及其理论] 08[工学] 0813[工学-建筑学] 071002[理学-动物学] 

基　　金：Supported by National Natural Science Foundation of China (30670898, 30572108) National Basic Research Program of China (973 Program) (2005CB522400) Capital Research Fund for Medical Development (2007-2040) 

主　　题：immune reconstitution α-galactosyleramide bone marrow transplantation 

摘      要：Objective To explore the effect of α-galactosyleramide （α-GalCer） on immune reconstitution under acute graft-versus-host disease （aGVHD）. Methods BALB/c mice were transplanted with allogeneic C57BL/6 bone marrow cells and splenocytes （both 1 × 10^7） after receiving lethal total-body irradiation, α-GalCer （100 ug/kg） or vehicle （dimethyl- sulfoxide） was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitufion, proliferation of T cells and B cells, hematopoiesis, and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3^＋, CD4^＋, CD8^＋, B220^＋, CD40＋, and CD86＋cells compared with the vehicle group. The number of colony forming unit per 1000 CD34^＋ cells in the et-GalCer group was higher than in the vehicle group （P=0.0012）. In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3^＋, CD4^＋, CD8^＋, and B220^＋ cells were higher in the α-GalCer group than in the normal group, especially the number of B220^＋ cells （P=0.007）. Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3^＋, CD4^＋, and CD8^＋ cells. Conclusion Administration of tl-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.