Genome-wide interrogation of transfer RNA-derived small RNAs in a mouse model of traumatic brain injury

作     者：Xiao-Jian Xu Meng-Shi Yang Bin Zhang Qian-Qian Ge Fei Niu Jin-Qian Dong Yuan Zhuang Bai-Yun Liu Xiao-Jian Xu;Meng-Shi Yang;Bin Zhang;Qian-Qian Ge;Fei Niu;Jin-Qian Dong;Yuan Zhuang;Bai-Yun Liu

作者机构：Beijing Key Laboratory of Central Nervous System InjuryBeijing Neurosurgical InstituteCapital Medical UniversityBeijingChina Beijing Key Laboratory of Central Nervous System Injury and Department of NeurosurgeryBeijing Neurosurgical Institute and Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Nerve Injury and Repair Center of Beijing Institute for Brain DisordersBeijingChina China National Clinical Research Center for Neurological DiseasesBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第2期

页      面：386-394页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China,Nos.81471238,81771327 Construction of Central Nervous System Injury Basic Science and Clinical Translational Research Platform,Budget of Beijing Municipal Health Commission 2020,No.PXM2020_026280_000002(all to BYL) 

主　　题：gene set enrichment analysis inflammation integrated analysis neurodegenerative disease next-generation sequencing secondary injury synaptic function transfer RNA-derived small RNAs transfer RNAs traumatic brain injury 

摘      要：Transfer RNA(t RNA)-derived small RNAs(ts RNAs) are a recently established family of regulatory small non-coding RNAs that modulate diverse biological processes. Growing evidence indicates that ts RNAs are involved in neurological disorders and play a role in the pathogenesis of neurodegenerative disease. However, whether ts RNAs are involved in traumatic brain injury-induced secondary injury remains poorly understood. In this study, a mouse controlled cortical impact model of traumatic brain injury was established, and integrated ts RNA and messenger RNA(m RNA) transcriptome sequencing were used. The results revealed that 103 ts RNAs were differentially expressed in the mouse model of traumatic brain injury at 72 hours, of which 56 ts RNAs were upregulated and 47 ts RNAs were downregulated. Based on micro RNA-like seed matching and Pearson correlation analysis, 57 differentially expressed ts RNA-m RNA interaction pairs were identified, including 29 ts RNAs and 26 m RNAs. Moreover, Gene Ontology annotation of target genes revealed that the significantly enriched terms were primarily associated with inflammation and synaptic function. Collectively, our findings suggest that ts RNAs may be associated with traumatic brain injury-induced secondary brain injury, and are thus a potential therapeutic target for traumatic brain injury. The study was approved by the Beijing Neurosurgical Institute Animal Care and Use Committee(approval No. 20190411) on April 11, 2019.