Presence of donor-and-recipient-derived DNA microchimerism in the cell-free blood samples of renal transplantation recipients associates with the acceptance of transplanted kidneys

作     者：Yao-Wen Fu Wei-Gang Wang Hong-Lan Zhou Lu Cai 

作者机构：Renal Transplantation Center of Jilin Provinceand Department of Urologythe China-Japan Union HospitalChangchun 130033China Department of Hematology and Oncologythe First Teaching HospitalJilin UniversityChangchun 130021China Departments of MedicineRadiation Oncologyand Pharmacology and Toxicologythe University of LouisvilleLouisvilleKentucky 40202USA 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2006年第8卷第4期

页      面：477-482,517页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：renal transplantation donor-and-recipient-derived DNA microchimerism immunotolerance renal rejection 

摘      要：Aim:To examine whether the existence of the donor-and recipient-derived DNA chimerism in recipient s plasma can be a predictive marker for the status of transplanted ***:One hundred and twenty-six female patients who had been transplanted with male kidneys were enrolled in the present *** these female recipients,the SRY_Ⅰ, DYZ_I^(Ⅰst)and DYZ_Ⅰ^(2nd)genes on the Y chromosome from the plasma were prospectively examined using reverse tran- scription polymerase chain reaction(RT-PCR).Results:SRY,DYZ_Ⅰ^(Ⅰst)and DYZ_Ⅰ^(2nd)sequences were detected in the cell-free blood(plasma)of 97(77%)of 126 female patients with male *** average time that the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients,*** frequency of the patients who had acute rejection after renal transplantation was ap- proximately 10% and 28% in microchimerism-positive and microchimerism-negative recipients,*** creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative ***:These results suggest that plasma DNA microchimerism present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys.(Asian J Androl 2006 Jul;8:477-482)