Altered profiles of fecal bile acids correlate with gut microbiota and inflammatory responses in patients with ulcerative colitis

作     者：Zhen-Huan Yang Fang Liu Xiao-Ran Zhu Fei-Ya Suo Zi-jun Jia Shu-Kun Yao 

作者机构：Graduate SchoolBeijing University of Chinese MedicineBeijing 100029China Department of GastroenterologyChina-Japan Friendship HospitalBeijing 100029China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2021年第27卷第24期

页      面：3609-3629页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by National Key Technology Support Program during“12th Five-Year Plan”Period of China,No.2014BAI08B00 National Key Research and Development Plan for Precision Medicine Research,No.2017YFC0910002 and Leapforward Development Program for Beijing Biopharmaceutical Industry(G20),No.Z171100001717008 

主　　题：Ulcerative colitis Gut microbiota Bile acids Takeda G-protein-coupled receptor 5 Vitamin D receptor 

摘      要：BACKGROUND Gut microbiota and its metabolites may be involved in the pathogenesis of inflammatory bowel *** clinical studies have recently shown that patients with ulcerative colitis(UC)have altered profiles of fecal bile acids(BAs).It was observed that BA receptors Takeda G-protein-coupled receptor 5(TGR5)and vitamin D receptor(VDR)participate in intestinal inflammatory responses by regulating NF-ĸB *** hypothesized that altered profiles of fecal BAs might be correlated with gut microbiota and inflammatory responses in patients with *** To investigate the changes in fecal BAs and analyze the relationship of BAs with gut microbiota and inflammation in patients with *** The present study used 16S rDNA sequencing technology to detect the differences in the intestinal flora between UC patients and healthy controls(HCs).Fecal BAs were measured by targeted metabolomics *** TGR5 and VDR expression was analyzed using immunohistochemistry,and serum inflammatory cytokine levels were detected by *** Thirty-two UC patients and twenty-three HCs were enrolled in this *** was found that the diversity of gut microbiota in UC patients was reduced compared with that in ***,Clostridium IV,Butyricicoccus,Clostridium XlVa,Faecalibacterium,and Roseburia were significantly decreased in patients with UC(P=3.75E-05,P=8.28E-07,P=0.0002,P=0.003,P=0.0003,and P=0.0004,respectively).Proteobacteria,Escherichia,Enterococcus,Klebsiella,and Streptococcus were significantly enriched in the UC group(P=2.99E-09,P=3.63E-05,P=8.59E-05,P=0.003,and P=0.016,respectively).The concentrations of fecal secondary BAs,such as lithocholic acid,deoxycholic acid,glycodeoxycholic acid,glycolithocholic acid,and taurolithocholate,in UC patients were significantly lower than those in HCs(P=8.1E-08,P=1.2E-07,P=3.5E-04,P=1.9E-03,and P=1.8E-02,respectively)and were positively correlated with Butyricicoccus,Roseburia,Clostridium IV,Faecalibacterium,and