Effects of lactose as an inducer on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia colirLTKA63 and rLTB

作     者：JieYan Shou-FengZhao Ya-EeiMao Yi-HuiLuo 

作者机构：DepartmentofMedicalMicrobiologyandParasitologyCollegeofMedicalScienceZhejiangUniversityHangzhou310031ZhejiangProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2004年第10卷第12期

页      面：1755-1758页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by the Excellent Young Teacher Fund of Ministry of Education of China and the General Science and Technology Research Plan of Zhejiang Province No.001110438 

主　　题：乳糖 诱导剂 基因表达 哈比特属 幽门菌 细菌感染 rUreB rHpaA 埃希氏菌属 rLTKA63 大肠菌 rLTB 重组细胞 

摘      要：AIM: To demonstrate the effect of lactose as an inducer on expression of the recombinant proteins encoded by Helicobacter pylori ureB and hpaA, and Escherichia coli LTB and LTKA63 genes and to determine the optimal expression parameters. METHODS: By using SDS-PAGE and BIO-RAD gel image analysis system, the outputs of the target recombinant proteins expressed by ***21, pET32a-hpaA-E, coliBL21, pET32a-L TKA63-E. coliBL21 and ***21 were measured when using lactose as inducer at different dosages, original bacterial concentrations, various inducing temperatures and times. The results of the target protein expression induced by lactose were compared to those by isopropyl-β-D-thiogalactoside (IPTG). The proteins were expressed in ***. RESULTS: Lactose showed higher efficiency of inducing the expression of rHpaA, rUreB, rLTB and rLTKA63 than IPTG. The expression outputs of the target recombinant proteins induced at 37℃ were remarkably higher than those at 28℃. Other optimal expression parameters for the original bacterial concentrations, dosages of lactose and inducing time were 0.8, 50 g/L and 4 h for rHpaA; 0.8, 100 g/L and 4 h for rLTKA63; 1.2, 100 g/L and 5 h for both rUreB and rLTB, respectively. CONCLUSION: Lactose, a sugar with non-toxicity and low cost, is able to induce the recombinant genes to express the target proteins with higher efficiency than IPTG. The results in this study establish a beneficial foundation for industrial production of Hpylorigenetic engineering vaccine.