Heat shock protein 20 promotes sirtuin 1-dependent cell proliferation in induced pluripotent stem cells

作     者：Mujib Ullah Nicole Pek Min Qian Gustavo Yannarelli Asma Akbar 

作者机构：Institute for Immunity and TransplantationStem Cell Biology and Regenerative MedicineSchool of MedicineStanford UniversityStanfordCA 94304United States Immunology and School of MedicineStanford UniversityStanfordCA 94304United States Laboratorio de Regulación Génica y Células MadreInstituto de Medicina TraslacionalTrasplante y Bioingeniería(IMeTTyB)Universidad Favaloro-CONICETBuenos Aires 1078Argentina Institute for Molecular MedicineSchool of MedicineStanford UniversityStanfordCA 94304United States 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2021年第13卷第6期

页      面：659-669页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：Heat shock proteins Stem cells Proliferation Induced pluripotent stem cells Sirtuin-1 Heat shock protein 20 Pluripotency 

摘      要：BACKGROUND Heat shock proteins(HSPs)are molecular chaperones that protect cells against cellular stresses or ***,it has been increasingly recognized that they also play crucial roles in regulating fundamental cellular ***20 has been implicated in cell proliferation,but conflicting studies have shown that it can either promote or suppress *** underlying mechanisms by which HSP20 regulates cell proliferation and pluripotency remain *** the effect of HSP20 on cell proliferation has been recognized,its role in inducing pluripotency in human-induced pluripotent stem cells(iPSCs)has not been *** To evaluate the efficacy of HSP20 overexpression in human iPSCs and evaluate the ability to promote cell *** purpose of this study was to investigate whether overexpression of HSP20 in iPSCs can increase pluripotency and *** We used iPSCs,which retain their potential for cell ***20 overexpression effectively enhanced cell proliferation and *** of HSP20 in iPSCs was characterized by immunocytochemistry staining and realtime polymerase chain *** also used cell culture,cell counting,western blotting,and flow cytometry analyses to validate HSP20 overexpression and its *** This study demonstrated that overexpression of HSP20 can increase the pluripotency in ***,by overexpressing HSP20 in iPSCs,we showed that HSP20 upregulated proliferation markers,induced pluripotent genes,and drove cell proliferation in a sirtuin 1(SIRT1)-dependent *** data have practical applications in the field of stem cell-based therapies where the mass expansion of cells is needed to generate large quantities of stem cell-derived cells for transplantation *** We found that the overexpression of HSP20 enhanced the proliferation of iPSCs in a SIRT1-dependent ***,we established the distinct crosstalk between HSP20 and SIR