Melatonin relieves heat-induced spermatocyte apoptosis in mouse testes by inhibition of ATF6 and PERK signaling pathways

作     者：De-Zhe Qin Hui Cai Cen He Dong-Hui Yang Jing Sun Wen-Lai He Ba-Lun Li Jin-Lian Hua Sha Peng De-Zhe Qin;Hui Cai;Chen He;Dong-Hui Yang;Jing Sun;Wen-Lai He;Ba-Lun Li;Jin-Lian Hua;Sha Peng

作者机构：College of Veterinary MedicineShaanxi Centre of Stem Cells Engineering&TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2021年第42卷第4期

页      面：514-524页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(32072815) General Project of the Key R&D Plan of Shaanxi Province(2019NY-091) Program of Shaanxi Province Science and Technology Innovation Team(2019TD-036) Fundamental Research Funds for the Central Universities(2452020157) 

主　　题：Heat stress ATF6 PERK Melatonin Spermatocyte 

摘      要：Normal spermatogenic processes require the scrotal temperature to be lower than that of the body as excessive heat affects spermatogenesis in the testes,reduces sperm quality and quantity,and even causes *** reticulum stress(ERS)is a crucial factor in many *** several studies have linked ERS to heat stress,researchers have not yet determined which ERS signaling pathways contribute to heat-induced testicular *** activates antioxidant enzymes,scavenges free radicals,and protects the testes from inflammation;however,few studies have reported on the influence of melatonin on heat-induced testicular *** a murine model of testicular hyperthermia,we observed that heat stress causes both ERS and apoptosis in the testes,especially in the *** observations were confirmed using the mouse spermatocyte cell line GC2,where the Atf6 and Perk signaling pathways were activated during heat *** of the above genes effectively reduced spermatocyte damage caused by heat *** with melatonin alleviated heat-induced apoptosis by inhibiting the Atf6 and Perk signaling *** mitigation was dependent on the melatonin *** vivo experiments verified that melatonin treatment relieved heat-induced testicular *** conclusion,our results demonstrated that ATF6 and PERK are important mediators for heat-induced apoptosis,which can be prevented by melatonin ***,our study highlights melatonin as a potential therapeutic agent in mammals for subfertility/infertility induced by testicular hyperthermia.