Protection of small-cell lung cancer circulating tumor cells by cellular fragmentation

作     者：Barbara Rath Adelina Plangger Doris Moser Maximilian Hochmair Ernst Ulsperger Gerhard Hamilton Barbara Rath;Adelina Plangger;Doris Moser;Maximilian Hochmair;Ernst Ulsperger;Gerhard Hamilton

作者机构：Department of Vascular SurgeryMedical University of ViennaVienna A-1190Austria Department of Cranio-Maxillofacial and Oral SurgeryMedical University of ViennaVienna A-1090Austria Hospital FloridsdorfVienna A-1210Austria Hospital HornHorn A-3580Austria 

出 版 物：《Journal of Cancer Metastasis and Treatment》 (癌症转移与治疗（英文版）)

年 卷 期：2020年第6卷第1期

页      面：345-354页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Small cell lung cancer circulating tumor cells shedding topotecan 

摘      要：Aim:Small-cell lung cancer(SCLC)disseminates aggressively and may exhibit high chemoresistance and poor survival *** this study,we aimed to investigate a new mechanism of drug resistance for SCLC circulating tumor cells(CTCs).Methods:SCLC CTC cell lines(n=4)which shed cellular fragments(MAT),as demonstrated by light and scanning electron microscopy,are compared to permanent SCLC *** proteins are detected by proteome arrays and the functional impact of MAT is studied using cytotoxicity tests involving cisplatin and ***:The SCLC CTC lines revealed layers of attached cellular fragments with a range of decreasing sizes from intact cells(approximately 12μm)down to small debris(approximately 2μm)which are not detectable in permanent SCLC *** SCLC CTC clusters represent cores of these fragment-coated *** profiling of MAT revealed a protein pattern similar to intact *** tests employing SCLC and SCLC CTC lines with chemotherapeutics used in therapy of SCLC demonstrated an inhibitory activity of MAT on the resulting ***:Generation of cell-associated debris by SCLC CTCs offers protective effects against cytotoxic drugs,representing a novel mechanism allowing survival of SCLC CTCs in patients.