Microarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer’s disease

作     者：Zhiqi Song Yajin Qu Yanfeng Xu Ling Zhang Li Zhou Yunlin Han Wenjie Zhao Pin Yu Yu Zhang Xianglei Li Chuan Qin Zhiqi Song;Yajin Qu;Yanfeng Xu;Ling Zhang;Li Zhou;Yunlin Han;Wenjie Zhao;Pin Yu;Yu Zhang;Xianglei Li;Chuan Qin

作者机构：Key Laboratory of Human Disease Comparative MedicineChinese Ministry of HealthBeijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijingChina 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2021年第4卷第3期

页      面：233-242页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100203[医学-老年医学] 10[医学] 

基　　金：CAMS Innovation Fund for Medical Sciences,Grant/Award Number:2016-12M-2-006 and 2019-I2M-1-003 Young Elite Scientists Sponsorship Program by CAST(YESS),Grant/Award Number:2019QNRC001 National Natural Science Foundation of China,Grant/Award Number:81901114 Fundamental Research Funds for the Central Universities,Grant/Award Number:3332019091。 

主　　题：5XFAD mouse model Alzheimer's disease biomarkers microarray miRNA urinary exosome 

摘      要：Background:Alzheimer s disease(AD)is an incurable and irreversible neurodegen-erative disease,without a clear pathogenesis.Therefore,identification of candidates before amyloid-βplaque(Aβ)deposition proceeds is of major significance for earlier intervention in AD.Methods:To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model,microRNAs(miRNAs)from urinary exosomes in 1-month-old pre-Aβaccumulation 5XFAD mice models and their littermate controls were profiled by mi-croarray analysis.The differentially expressed miRNAs were further analyzed via droplet digital PCR(ddPCR).Results:Microarray analysis demonstrated that 48 differentially expressed miRNAs(18 upregulated and 30 downregulated),of which six miRNAs-miR-196b-5p,miR-339-3p,miR-34a-5p,miR-376b-3p,miR-677-5p,and miR-721-were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR.Conclusions:Urinary exosomal miRNAs showing differences in expression prior to Aβ-plaque deposition were identified.These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications.