Click chemistry-based pre-targeting cell delivery for cartilage regeneration

作     者：Cynthia M.Co Samira Izuagbe Jun Zhou Ning Zhou Xiankai Sun Joseph Borrelli Liping Tang 

作者机构：Department of BioengineeringUniversity of Texas at ArlingtonPO Box 19138ArlingtonTX 76019USA Department of RadiologyUniversity of Texas Southwestern MedicalDallasTX 75390USA 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第3期

页      面：11-21页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This work was partially supported by UTA Research&Scholarship Excellence Gift The authors acknowledge the financial support from the National Heart,Lung,and Blood Institute[NIH T32 HL134613 to C.C.] The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH 

主　　题：chondrocyte cartilage regeneration cell therapy click chemistry 

摘      要：A fraction of the OA patient population is affected by post-traumatic osteoarthritis(PTOA)following acute joint *** or reversing the progression of PTOA following joint injury could improve long-term functional outcomes,reduced disability,and medical *** more effectively treat articular cartilage injury,we have developed a novel cell-based therapy that involves the pretargeting of apoptotic chondrocytes and the delivery of healthy,metabolically active chondrocytes using click ***,a pre-targeting agent was prepared via conjugating apoptotic binding peptide(ApoPep-1)and trans-cyclooctene(TCO)onto polyethylene glycol(PEG)polymer *** pre-targeting agent would be introduced to injured areas of articular cartilage,leading to the accumulation of TCO groups on the injured areas from actively binding to apoptotic ***,methyltetrazine(Tz)-bearing chondrocytes would be immobilized on the surface of TCO-coated injured cartilage via Tz-TCO click chemistry *** an ex vivo human cartilage explant PTOA model,the effectiveness of this new approach was *** studies show that this novel approach(Tz-TCO click chemistry)significantly enhanced the immobilization of healthy and metabolically active chondrocytes to the areas of apoptotic *** analyses demonstrated that this treatment regimen would significantly reduce the area of cartilage degeneration and enhance ECM *** results support that Tz-TCO click chemistry-mediated cell delivery approach has great potential in clinical applications for targeting and treatment of cartilage injury.