Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin depolymerizing factor 1 on microfilaments in okra
作者机构：Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignCollege of ForestryBeijing Forestry UniversityBeijing 100000China Key Laboratory of Forest Plant EcologyMinistry of EducationNortheast Forestry UniversityHarbin 150000China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：2227-2240页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 071001[理学-植物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）]
基 金：The auth ors gratefully ack no wledge the fi nan cial supp ort of the"Nati onal Key R&D Program of China"(2019YFD1000605-1) "The National Natural Science Foun dati on of Ch ina"(31922058) "Outsta nding Young Tale nt Fund in Beij ing Forestry University"(2019JQ03009) "The National Natural Science Foundation of China"(31800509),(31901281),(31930076) "The 111 Project"(B20088) "Heilongjiang Touyan Innovation Team Program"(Tree Genetics and Breeding Innovation Team)
主 题：effect polymerization polymer
摘 要：Mature pollen germinates rapidly on the stigma,extending its pollen tube to deliver sperm cells to the ovule for *** success of this process is an important factor that limits *** flavonoid content increased signi ficantly during pollen germination and pollen tube growth,which suggests it may play an important role in these ***,the speci fi c mechanism of this involvement has been little *** previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus(okra),but its speci fic mechanism is still ***,in this study,we focused on the effect of hyperoside in regulating the actin-depolymerizing factor(ADF),which further affects the germination and growth of *** found that hyperoside can prolong the effective pollination period of okra by 2-3-fold and promote the growth of pollen tubes in the ***,we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular micro fi *** can promote pollen germination and pollen tube elongation in ***,AeADFl was identi fied out of all AeADF genes as being highly expressed in pollen tubes in response to *** addition,hyperoside promoted AeADF1-mediated micro filament dissipation according to micro filament severing experiments in *** the pollen tube,the gene expression of AeADFl was reduced to 1/5 by oligonucleotide *** decrease in the expression level of AeADFl partially reduced the promoting effect of hyperoside on pollen germination and pollen tube *** research provides new research directions for flavonoids in reproductive development.
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