Anti-inflammatory Activity and Mechanism of Total Flavonoids from the Phloem of Paulownia elongate S.Y. Hu in LPS-stimulated RAW264.7 Macrophages

作     者：Liyan LI Tao HUANG Chong LAN 

作者机构：Medical SchoolHuanghe Science&Technology UniversityZhengzhou 450063China Zhengzhou Key Laboratory of Drug Screening and Activity EvaluationHuanghe Science&Technology UniversityZhengzhou 450063China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2021年第10卷第3期

页      面：117-120,124页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Henan Provincial Department of Science&Technology(172102310165) Henan Provincial Department of Education(21B350001) 

主　　题：Paulownia elongate Total flavonoids Anti-inflammatory activity Mechanisms 

摘      要：The juice leaked from broken phloem of Paulownia elongate *** has been used to cure acute inflammation resulted from stung injury by poisonous insects for a long time in China,but its potential mechanism remains *** present study was designed to evaluate anti-inflammatory activity and mechanism of total flavonoids from the phloem of *** *** in RAW264.7 ***(LPS)-induced nitric oxide(NO)was measured by Griess and mRNA of pro-inflammatory mediators was analyzed by *** viability was measured using cell counting kit(CCK)-8 *** protein level was analyzed by Western *** results showed that the total flavonoids of *** significantly inhibited the production of the pro-inflammatory mediators such as NO,interleukin(IL)-1β and interleukin(IL)-6 in LPS-stimulated RAW264.7 *** flavonoids of *** exerted potential anti-inflammatory activity through the regulation of several signaling *** flavonoids of *** inhibited JAK/STAT by blocking JAK2 and STAT3 phosphorylation *** is the first report on anti-inflammatory activity of total flavonoids from the phloem of ***,which suggests that the total flavonoids of *** may have great potential for the development of anti-inflammatory drug to treat inflammatory disorders.