Metabolomics analysis of poly(L-lactic acid)nanofibers’performance on PC12 cell differentiation

作     者：Xiaoman Su Yan Huang Rong Chen Yiwen Zhang Meichen He Xiaoying Lu 

作者机构：State Key Laboratory of BioelectronicsSchool of Biological Science and Medical EngineeringSoutheast University2#Si PailouNanjing 210096China Department of ResearchSQ Medical Device Co.Ltd17#Xinghuo RoadNanjing 211500China Coinnovation Center of NeuroregenerationNantong UniversityNantongJiangsu Province 226019China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第4期

页      面：72-87页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0714[理学-统计学（可授理学、经济学学位）] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：This study received the support of the National Natural Science Foundation of China(31170910 31971254 and 31271012) 

主　　题：nanofibers metabolomics and pathway analysis PC12 cell differentiation molecular mechanism 

摘      要：The aim of this article is to reveal the influence of aligned/random poly(L-lactic acid)(PLLA)nanofibers on PC12 cell differentiation from the perspective of metabolic ***,three materials-PLLA aligned nanofibers(PLLA AF),PLLA random nanofibers(PLLA RF)and PLLA films(control)-were prepared by electrospinning and spin *** surface morphologies were ***,the cell viability,cell morphology and neurite length of PC12 cells on the surface of the three materials were evaluated,indicating more neurites in the PLLA RF groups but the longer average neurite length in the PLLA AF ***,the metabolite profiles of PC12 cells cultured on the surface of the three nanofibers after 12 h,24 h and 36 h showed that,compared with the control,51,48 and 31 types of differential metabolites were detected at the three time points among the AF groups,respectively;and 56,45 and 41 types among the RF groups,***,the bioinformatics analysis of differential metabolites identified two pathways and three metabolites critical to PC12 cell differentiation influenced by the *** addition,the verification experiment on critical metabolites and metabolic pathways were *** integrative analysis combining cytology,metabolomics and bioinformatics approaches revealed that though both PLLA AF and RF were capable of stimulating the synthesis of neurotransmitters,the PLLA AF were more beneficial for PC12 cell differentiation,whereas the PLLA RF were less effective.