Neuroprotective Effects of San-Jia-Fu-Mai Decoction: Studies on the in vitro and in vivo Models of Parkinson's Disease

作     者：Chang Qu Ling Liu Qing-Qing Xu Yan-Fang Xian Zhi-Xiu Lin Chang Qu;Ling Liu;Qing?Qing Xu;Yan?Fang Xian;Zhi?Xiu Lin

作者机构：School of Chinese MedicineFaculty of MedicineThe Chinese University of Hong Kong School of Basic MedicineGuizhou University of Traditional Chinese MedicineGuizhouChina Brain Research CentreSchool of Chinese MedicineFaculty of MedicineThe Chinese University of Hong Kong Hong Kong Institute of Integrative MedicineFaculty of MedicineThe Chinese University of Hong KongShatinNTHong Kong 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2021年第7卷第2期

页      面：192-200页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Innovation and Technology Commission Funding Administrative System of Hong Kong(Project No.:ITS/122/19FP) the Natural Science Fund of Guangdong(project no.2019A1515011257) 

主　　题：1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 1-methyl-4-phenylpyridinium motor function deficits oxidative stress Parkinson’s disease San-Jia-Fu-Mai decoction 

摘      要：Objective:The objective of this study was to investigate whether 70%aqueous ethanol extract of San-Jia-Fu-Mai decoction extract(SJFMDE)could protect against 1-methyl-4-phenylpyridinium(MPP+)-induced oxidative stress in PC12 cells and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced motor function deficits in *** and Methods:The cell viability,the levels of intracellular reactive oxygen species(ROS),malondialdehyde(MDA),and glutathione(GSH)in the MPP+-treated PC12 cells were *** function deficits and dopamine(DA)level in the brain striatum and tyrosine hydroxylase(TH)-positive cells in substantia nigra pars compacta(SNc)of the MPTP-treated mice were ***:The results showed that SJFMDE could reduce cell death and the levels of ROS and MDA while increase the level of GSH in the MPP+-treated PC12 *** addition,in vivo studies showed that oral administration of SJFMDE(3,6,and 12 g/kg)significantly improved the motor function deficits induced by MPTP and enhanced the DA level in the striatum and TH-positive neuronal cells in SNc of the MPTP-treated ***:Our results revealed that SJFMDE possessed neuroprotective effects against neurotoxicity induced by MPP+and motor function deficits induced by MPTP via suppressing oxidative stress and increasing the levels of DA and TH,indicating that SJFMDE might be a promising Chinese medicine formula for the treatment of Parkinson’s disease.